ArticleAnandamide regulates lipopolysaccharide-induced nitric oxide synthesis and tissue damage in the murine uterus
Section snippets
Ana Franchi received her initial training in chemistry at the School of Sciences, University of Buenos Aires. She first became interested in prostaglandin metabolism in the sex cycle. As a post-doc she began to work in pregnancy and she developed two rodent models of diseases that take place in pregnancy: pre-term labor and septic abortion. She is currently a researcher for the National Research Council and a director of CEFYBO (School of Medicine).
References (33)
- et al.
Biosynthesis, uptake, and degradation of anandamide and palmitoylethanolamide in leukocytes
Journal of Biological Chemistry
(1997) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Analytical Biochemistry
(1976)- et al.
Quantification of nitrate and nitrite in extracellular fluids
Methods in Enzymology
(1996) - et al.
N-Acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation
Journal of Biological Chemistry
(2005) - et al.
Lipopolysaccharide induces anandamide synthesis in macrophages via CD14/MAPK/phosphoinositide 3-kinase/NF-κB independently of platelet-activating factor
Journal of Biological Chemistry
(2003) Prediction of pregnancy loss
Lancet
(2000)- et al.
Lipopolysaccharide downregulates fatty acid amide hydrolase expression and increases anandamide levels in human peripheral lymphocytes
Archives of Biochemistry and Biophysics
(2001) - et al.
Anandamide uptake by human endothelial cells and its regulation by nitric oxide
Journal of Biological Chemistry
(2000) - et al.
Relation between decreased anandamide hydrolase concentrations in human lymphocytes and miscarriage
Lancet
(2000) - et al.
Endocannabinoids
European Journal of Pharmacology
(1998)
Ligand–receptor signaling with endocannabinoids in preimplantation embryo development and implantation
Chemistry and Physics of Lipids
Inhibition of nitric oxide synthesis potentiates apoptosis in the rabbit corpus luteum
Reproductive BioMedicine Online
Synthesis of prostaglandin E2 ethanolamide from anandamide by cyclooxygenase-2
Journal of Biological Chemistry
Nitric oxide mediates prostaglandin deleterious effect on lipopolysaccharide-triggered murine fetal resorption
Proceedings of the National Academy of Sciences of the United States of America
Role of nitric oxide in implantation and menstruation
Human Reproduction
Synergistic role of nitric oxide and progesterone during the establishment of pregnancy in the rat
Human Reproduction
Cited by (28)
Maternal obesity reverses the resistance to LPS-induced adverse pregnancy outcome and increases female offspring metabolic alterations in cannabinoid receptor 1 knockout mice
2021, Journal of Nutritional BiochemistryCitation Excerpt :Whereas low levels of anandamide are required for implantation and trophoblast outgrowth, elevated anandamide concentrations lead to pregnancy failure [49,50]. We have previously shown that CB1 receptor activation mediates the deleterious effects of lipopolysaccharide (LPS) on nitric oxide and prostaglandin production, and subsequent tissue damage during early embryonic loss [51–53]. Similarly, it has been shown that the eCS induces apoptosis in rat decidual cells [54,55].
Impact of Endocannabinoid System Manipulation on Neurodevelopmental Processes Relevant to Schizophrenia
2021, Biological Psychiatry: Cognitive Neuroscience and NeuroimagingCitation Excerpt :It modulates fundamental gestational events and is crucial for the proper initiation of parturition (37–48). The ECS also participates in the modulation of MIA adverse effects on fetal brain development and birth outcome (49–53). Based on the observation that CB2Rs are mainly located on immune cells and brain glial cells (54–58), it is tempting to speculate that changes in CNR2 and subsequent alterations in CB2Rs could have a role in MIA-mediated developmental abnormalities.
Lipopolysaccharide-induced murine embryonic resorption involves changes in endocannabinoid profiling and alters progesterone secretion and inflammatory response by a CB1-mediated fashion
2015, Molecular and Cellular EndocrinologyCitation Excerpt :The endocannabinoids AEA and 2-AG are ligands for the cannabinoid receptors CB1 which is highly expressed in the human endometrium (Taylor et al., 2010a) and it has been shown to play an important role in oviductal embryo transport (Wang et al., 2004) and uterine embryo receptivity (Paria et al., 2001). Since local uterine AEA levels are important not only for a normal pregnancy but also to participate in LPS-induced tissue damage (Vercelli et al., 2009a, 2009b, 2012), we hypothesized that CB1-KO mice would be resistant to LPS-induced embryo loss. Indeed, LPS administration (0.5 or 1 µg/g of weight) to CB1-KO mice resulted in fewer EmR when compared to WT mice, with the embryo loss rate of the lower dose being no different from vehicle.
Progesterone reverts LPS-reduced FAAH activity in murine peripheral blood mononuclear cells by a receptor-mediated fashion
2013, Molecular and Cellular EndocrinologyCitation Excerpt :Moreover, LPS-induced EmRe is associated with an increased uterine and decidual production of nitric oxide (NO) and prostaglandins (PGs), two molecules that play key roles in this process (Ogando et al., 2003; Aisemberg et al., 2007). Furthermore, our previous work suggests that LPS could augment AEA levels in uterine explants by inhibiting its degradation Indeed, we demonstrated that AEA regulate LPS-induced NO production and tissue damage in the uterus of pregnant mice (Vercelli et al., 2009). In addition, Maccarrone et al. (2001b) showed that LPS downregulates FAAH expression and increases AEA levels in human peripheral lymphocytes while Liu et al. showed that LPS induces AEA but not 2-AG in murine macrophages (Liu et al., 2003).
Differential expression of endocannabinoid system in normal and preeclamptic placentas: Effects on nitric oxide synthesis
2013, PlacentaCitation Excerpt :The radioactivity was measured by liquid scintillation counting. Protein concentration was measured by the Bradford assay [23]. Enzyme activity is reported as fmol of [14C]-citrulline produced by mg of protein during 15 min.
Cyclooxygenase-2 prostaglandins mediate anandamide-inhibitory action on nitric oxide synthase activity in the receptive rat uterus
2012, European Journal of Pharmacology
Ana Franchi received her initial training in chemistry at the School of Sciences, University of Buenos Aires. She first became interested in prostaglandin metabolism in the sex cycle. As a post-doc she began to work in pregnancy and she developed two rodent models of diseases that take place in pregnancy: pre-term labor and septic abortion. She is currently a researcher for the National Research Council and a director of CEFYBO (School of Medicine).