Myocardial glucose uptake after dobutamine stress in chronic hibernating swine myocardium☆
Section snippets
Methods
This study was performed under the guidance of the animal care committee at the VA Medical Center (Minneapolis, Minn) and conforms with the US National Institutes of Health “Guide for the Care and Use of Laboratory Animals” (publication No. 85-23, revised 1996).
Regional myocardial function at baseline
During the early study, systolic wall thickening at baseline was similar in the LAD and remote regions. During the late study, an anterior wall motion abnormality was evident and systolic wall thickening in the LAD region at baseline was lower than in the remote region (P < .05) (Figure 1). During the initial echocardiographic study, end-diastolic wall thickness in the LAD and remote regions was 0.78 ± 0.05 cm and 0.77 ± 0.06 cm, respectively (P = not significant [NS]). During the final
Discussion
The principal findings of this study are as follows. First, myocardial FDG uptake in chronic hibernating myocardium is higher than in remote regions after an infusion of dobutamine, and the increased glucose metabolic activity is proportional to the relative impairment in dobutamine-induced blood flow. Second, the abnormalities in poststress FDG uptake are associated with changes in GLUT4, including increased total content by immunoblotting and enhanced translocation by immunohistochemistry.
Acknowledgements
The authors have indicated they have no financial conflicts of interest.
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This study was supported in part by General Medical Research funds (E.O.M.) from the US Department of Veterans Affairs.