Ethanol effects on lipid peroxidation and glutathione-mediated defense in rat small intestine: Role of dietary fats

doi:10.1016/S0741-8329(97)00099-2

Alcohol

Volume 15, Issue 1, January 1998, Pages 65-69

https://doi.org/10.1016/S0741-8329(97)00099-2 Get rights and content

Abstract

The effect of ethanol feeding for 5 weeks on lipid peroxidation status of small intestine was studied in rats maintained on either a rat pellet (RP) or a semisynthetic diet containing coconut oil (CCO), corn oil (CO), or fish oil (FO). Highest rate of iron/ascorbate-induced lipid peroxidation was observed in intestinal mucosa of FO-fed rats, which was further elevated (p < 0.05) upon ethanol administration. Purified brush borders from all the ethanol-treated dietary groups were more susceptible to iron-induced lipid peroxidation. Level of nonprotein thiols was increased by ethanol feeding to rats given CO or FO. FO-fed rats exhibited increased activities of glutathione reductase (GR), glutathione-S-transferase (GST), and catalase (Cat). Glutathione peroxidase (GPx) was the lowest in the CCO group. Ethanol-treated FO group exhibited increased GST and GPx activities compared to controls, whereas in rats fed the RP or CO diet, ethanol feeding significantly decreased GST activity. GR and Cat activities were not affected under these conditions. Thus, ethanol exposes the small intestinal mucosa to oxidative stress. The effects were more pronounced in rats fed n-3 fatty acid-rich (FO) diet. The corresponding rise in GPx and GST levels may reflect the adaptive changes in intestine.

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ArticleEthanol effects on lipid peroxidation and glutathione-mediated defense in rat small intestine: Role of dietary fats

Abstract

Lancet

Biochem. Pharmacol.

Biochem. Biophys. Acta

Gastroenterology

J. Biol. Chem.

J. Nutr.

Gastroenterology

J. Biol. Chem.

J. Lipid Res.

Biochim. Biophys. Acta

Anal. Biochem.

Alcohol

Cancer Lett.

Biochim. Biophys. Acta

Acute exposure of small intestine to ethanol. Effects on morphology and function

Dig. Dis. Sci.

Microsomal lipid peroxidation

Methods Enzymol.

Reduced hepatic α-tocopherol content after long-term administration of ethanol to rats

Biochim. Biophys. Acta

Dietary modulation of intestinal plasma membrane structure and function

Effect of fish oil and coconut oil on antioxidant defense system and lipid peroxidation in rat liver

Free Radic. Res. Commun.

Article
Ethanol effects on lipid peroxidation and glutathione-mediated defense in rat small intestine: Role of dietary fats