Distribution and postnatal ontogeny of adenosine A2A receptors in rat brain: comparison with dopamine receptors
Section snippets
Animals
These studies, which were approved by the regional animal ethics committee, used Sprague–Dawley rats (B&K, Sollentuna, Sweden. The number of animals at each age group was (figures within parenthesis): Newborn (19 animals), two-day-old (6), five-day-old (6), seven-day-old (15), 10-day-old (6), 15-day-old (5), 21-day-old (5) and adult (three- to four-months-old; 21 animals). Three to five animals of each age from at least two separate litters were used for each experiment, unless specifically
[3H]CGS 21680, [3H]SCH 23390 and [3H]raclopride binding in adults
As seen in Fig. 1 and Fig. 2, and in agreement with previous studies, A2A receptors, D1 receptors and D2 receptors were enriched in caudate–putamen, accumbens nucleus, olfactory tubercle and globus pallidus. However, at all ages there was also a lower level of binding of [3H]CGS 21680 to many of the brain's gray matter structures including the cerebral cortex and hippocampus, confirming recent results.48, 49There was also binding to the olfactory bulb (see below). In agreement with previous
Usefulness of the radioligands used
In the present study we have used radioligands reported to be quite selective for D1, D2, A2A, and A1 receptors, respectively. Although the binding of [3H]CGS 21680 to the basal ganglia structures in focus here appear to represent classical A2A receptors (as evidenced by the pharmacology), we have previously found that in cortical areas [3H]CGS 21680 appears to bind to a site different from the A2A receptor present in the striatum.14, 48, 49The present results show that these low-affinity
Conclusions
In the areas examined, A1, A2A, D1 and D2 receptors increase markedly during the first weeks of extrauterine life, as reported for many receptors.[41]We have found that quantitative receptor autoradiography is a very useful technique to study ontogeny of brain receptors, since it gives highly reproducible values for small regions in the developing animal that are difficult to dissect out and from which only minute amounts of membrane protein can be prepared. Autoradiography and regional
Acknowledgements
This study was supported by the Swedish Medical Research Council (proj. no. 2553), Magnus Bergvall's and Lars Hierta's foundations, the Swedish Society for Medical Research, the Institute for Scientific Information on Coffee, Astra Arcus, a BIOMED 2 project (BM4-CT96-0238), and Karolinska Institutet. B.J. was a recipient of a Swedish MRC scholarship. V.G. was supported by the European Science Foundation, an ENP/European Research Grant and grants from the Swedish Institute and the Karolinska
References (110)
- et al.
Regional distribution of dopamine D1 and D2 receptors in the nucleus accumbens of the rat
Brain Res.
(1989) - et al.
Autoradiographic localization of D1 and D2 receptors in rat nucleus accumbens: resistance to differential rearing conditions
Neuroscience
(1991) - et al.
Characterization of the binding of 3H-SCH 23390, a selective D-1 receptor antagonist ligand, in rat striatum
Life Sci.
(1984) - et al.
A detailed mapping of dopamine D-2 receptors in rat central nervous system by autoradiography with [125I]iodosulpiride
Neuroscience
(1987) - et al.
Localization of dopamine D3 receptor mRNA in the rat brain using in situ hybridization histochemistry: comparison with dopamine D2 receptor mRNA
Brain Res.
(1991) - et al.
Postnatal development of striatal dopamine function. I. Examination of D1 and D2 receptors, adenylate cyclase regulation and presynaptic dopamine markers
Devl Brain Res.
(1990) - et al.
Comparative development of D1-dopamine and μ opiate receptors in normal and 6-hydroxydopamine-lesioned neonatal rat striatum: dopaminergic fibers regulate μ but not D1 receptor distribution
Devl Brain Res.
(1991) - et al.
Ontogenetic expression of D2 dopamine receptor mRNA in rat corpus striatum
Devl Brain Res.
(1991) - et al.
Dopamine receptors in the human brain: autoradiographic distribution of D1 sites
Neuroscience
(1989) - et al.
Evidence for functionally important adenosine A2a receptors in the rat hippocampus
Brain Res.
(1994)
Quantitative autoradiographic study of the postnatal development of adenosine A1 receptors and their coupling to G-proteins in the rat brain
Neuroscience
D-1 dopamine receptors in the rat brain. Autoradiographic localization using [3H]SCH 23390
Eur. J. Pharmac.
The D1 dopamine receptor in the rat brain: quantitative autoradiographic localization using an iodinated ligand
Neuroscience
Bicuculline- and allylglycine-induced epilepsy in the developing rat
Expl Neurol.
Radioautographic method for quantifying regional monoamine innervations in the rat brain. Application to the cerebral cortex
Brain Res.
Purinergic mechanisms in epilepsy
Prog. Neurobiol.
Autoradiographic distribution of the D1 agonist [3H]SKF 38393, in the rat brain and spinal cord. Comparison with the distribution of D2 dopamine receptors
Neuroscience
Heterogenous distribution of dopamine D2 receptors within the rat striatum as revealed by autoradiography of [3H]N-n-propylnorapomorphine binding sites
Neurosci. Lett.
The distribution of adenosine A1-receptors and 5′-nucleotidase in the brain of some commonly used experimental animals
Neuroscience
Normal and abnormal determinants of dopamine receptor ontogeny in the central nervous system
Prog. Neurobiol.
Adenosine-dopamine interactions in the brain
Neuroscience
Molecular cloning of a rat A2 adenosine receptor: selective co-expression with D2 dopamine receptors in rat striatum
Molec. Brain Res.
Autoradiographic localization of [3H]quinpirole binding to dopamine D2 and D3 receptors in rat brain
Eur. J. Pharmac.
Ontogenesis of adenosine receptors in the central nervous system of the rat
Devl Brain Res.
Postnatal development of dopamine D1 and D2 receptor sites in rat striatum
Devl Brain Res.
Developmental and age-related changes in D1-dopamine receptors and dopamine content of rat striatum
Devl Brain Res.
Neurotransmitters and neuromodulators in the basal ganglia
Trends Neurosci.
D2 dopamine receptor gene expression in the rat striatum during ontogeny: an in situ hybridization study
Devl Brain Res.
Neonatal exposure to therapeutic caffeine concentrations alters the ontogeny of adenosine A1 receptors in brain of rats
Neuropharmacology
Raclopride, a new selective ligand for the dopamine-D2 receptors
Prog. Neuro-Psychopharmac. biol. Psychiat.
Tritium quench in autoradiography during postnatal development of rat forebrain
Brain Res.
Postnatal ontogeny of the rat brain neurotensin receptor mRNA
Neurosci. Lett.
The A2-selective adenosine analog, CGS 21680, depresses locomotor activity but does not block amygdala kindled seizures in rats
Neurosci. Lett.
Direct autoradiographic localization of adenosine A2 receptors in the rat brain using the A2-selective agonist [3H]CGS 21680
Eur. J. Pharmac.
Effects of mono- and divalent ions on the binding of the adenosine analogue CGS 21680 to adenosine A2 receptors in rat striatum
Biochem. Pharmac.
The binding of the adenosine A2 receptor selective agonist [3H]CGS 21680 to rat cortex differs from its binding to rat striatum
Eur. J. Pharmac.
Further characterization of the binding of the adenosine receptor agonist [3H]CGS 21680 to rat brain using autoradiography
Neuropharmacology
Striatal topography of D2 receptors correlates with indexes of cholinergic neuron localization
Neurosci. Lett.
Impaired ontogeny of striatal dopamine D1 and D2 binding sites after postnatal treatment of rats with SCH-23390 and spiroperidol
Devl Brain Res.
Quantitative receptor mapping by autoradiography: some current technical problems
Trends Neurosci.
Dopamine D4 versus D2 receptor selectivity of dopamine receptor antagonists: possible therapeutic implications
Eur. J. Pharmac.
D2 dopamine receptor gene expression by cholinergic neurons in the rat striatum
Neurosci. Lett.
Postnatal development of D1 dopamine receptors in the medial prefrontal cortex, striatum, and nucleus accumbens of normal and neonatal 6-hydroxydopamine treated rats: a quantitative autoradiographic analysis
Devl Brain Res.
Mosaic-like distribution of dopamine receptors in rat neostriatum and its relationship to striosomes
Brain Res.
A comparison of D1 receptor binding and mRNA in rat brain using receptor autoradiographic and in situ hybridization techniques
Neuroscience
Effects of chronic caffeine on brain adenosine receptors: regional and ontogenetic studies
Life Sci.
Adenosine A2 receptors: selective localization in the human basal ganglia and alterations with disease
Neuroscience
Distribution of D5 dopamine receptor mRNA in rat brain
Neurosci. Lett.
Prenatal haloperidol alters striatal dopamine and opiate receptors
Brain Res.
Increased seizure susceptibility of the immature brain
Devl Brain Res.
Cited by (0)
- 1
Present address: Department of Experimental Pharmacology, Institute of Physiology, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.