Alpha-lipoic acid prevents ethanol-induced protein oxidation in mouse hippocampal HT22 cells

doi:10.1016/S0304-3940(02)00415-9

Neuroscience Letters

Volume 328, Issue 2, 9 August 2002, Pages 93-96

https://doi.org/10.1016/S0304-3940(02)00415-9 Get rights and content

Abstract

Oxidative stress is involved in a number of neurological disorders, including the neurotoxic effects of ethanol. Recent studies have described a neuroprotective potential of α-lipoic acid (LC) in several models of neuronal cell death related to oxidative stress. We tested the hypothesis that LC could be effective in preventing ethanol-induced neurotoxicity employing the clonal hippocampa cell line HT22. A 24 h incubation with ethanol 100–600 mM caused a dose-dependent loss of cell viability and a significant increase of the overall intracellular protein oxidation. Coincubation with LC 0.1 mM resulted in a significant decrease of ethanol-related neurotoxicity and a complete prevention of the ethanol-induced intracellular protein oxidation. These results indicate that the radical scavenging properties of LC are effective to ameliorate ethanol-induced neurotoxicity.

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Acknowledgements

MP and TG were supported by the Else-Kröner-Fresenius-Stiftung, Bad Homburg, Germany. TG was supported by DFG.

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Alpha-lipoic acid prevents ethanol-induced protein oxidation in mouse hippocampal HT22 cells

Abstract

Section snippets

Acknowledgements

Neurosci. Lett.

Eur. J. Pharm. Sci.

Free Radic. Biol. Med.

Free Radic. Biol. Med.

Brain Res.

Alcohol

Free Rad. Biol. Med.

Neurosci. Lett.