Elsevier

Neuroscience Letters

Volume 286, Issue 2, 2 June 2000, Pages 111-114
Neuroscience Letters

γ-Aminobutyric acid enhancement of halothane binding in rat cerebellum

https://doi.org/10.1016/S0304-3940(00)01105-8Get rights and content
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Abstract

Quantitative autoradiography of 14C-halothane direct photolabeled rat cerebellum sections was performed in the presence of increasing concentrations of γ-aminobutyric acid (GABA) or glutamate to test the hypothesis that a coupled binding equilibrium between the anesthetic and neurotransmitter exists. The results show that halothane binding was enhanced in the presence of GABA by ∼50% in the molecular layer and to a lesser extent in the granular layer, with no change in the myelin layer. Glutamate, however, did not enhance halothane binding in any layer. These data confirm the presence of coupling, and thus suggest a direct interaction of halothane with a GABA binding protein.

Keywords

Autoradiography
Anesthetics
Agonist
Photolabeling
Glutamate
γ-Aminobutyric acid
Receptors

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