Several Receptors Mediate the Antisecretory Effect of Peptide YY, Neuropeptide Y, and Pancreatic Polypeptide on VIP-Induced Fluid Secretion in the Rat Jejunum In Vivo
Section snippets
Animals
Male Wistar rats weighing 180–200 g (Iffa-Credo, Les Oncins, F 69210 L'Arbresle) were fasted for 24 h with free access to water before the experiments.
Peptides
PYY(1–36) (pig, rat), NPY(1–36) (human, rat), PP (human), PYY(3–36), PYY(13–36), NPY(3–36), NPY (13–36), NPY(22–36), [Leu31,Pro34]PYY, [Leu31,Pro34] NPY, and VIP (human, pig, rat) were purchased from Neosystem, Strasbourg France. PYY(22–36) and N-α-AcPYY(22–36) were generous gifts of A. Balasubramaniam (University of Cincinnati Medical Center,
Effect of PYY, NPY, and PP on VIP-Stimulated Fluid Jejunal Secretion
PYY(1–36) inhibited in a dose-related manner VIP-stimulated jejunal fluid secretion. In preliminary experiments, two different doses of VIP were used. In basal conditions, the mean water net flux was −46.9 ± 3.4 μl/cm/30 min, indicating a basal absorption. After stimulation by 100 μg/kg/h of VIP, the jejunal secretion reached 39 ± 2.7 μl/cm/30 min; the inhibition was complete after 300 pmol/kg of PYY and the ID50 was 30 pmol/kg of PYY (not shown). After stimulation by 30 μg/kg/h of VIP, the
Discussion
The present study was designed to investigate the subtypes of Y receptors involved in the jejunal antisecretory effect of PYY and NPY by comparing the effects of the full-length peptides with different fragments or analogues on VIP-stimulated jejunal secretion in the rat in vivo.
The model chosen for the present work was the jejunal ligated loop in situ in anesthetized rats. In a previous article ([43]), we showed that this model was appropriate to evidence the antisecretory effect of PYY and
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