IL-2 enhances allergic airway responses in rats by increased inflammation but not through increased synthesis of cysteinyl leukotrienes☆,☆☆,★
Section snippets
Animals and sensitization
Fifty-nine male highly inbred BN rats (Harlan Sprague Dawley) that were 7 to 9 weeks old were studied. All rats were sensitized with OVA by subcutaneous injection of 1 mL of sterile solution containing 1 mg of OVA and 3.5 mg of Al(OH) 3 gel in 1 mL of saline. On the same occasion, 0.5 mL of Bordetella pertussis vaccine containing 6 × 10 9 heat-killed bacilli was injected intraperitoneally into all of the rats except the 18 that underwent studies with lung lavage.
Drugs and chemicals
IL-2 was graciously provided by
Effect of IL-2 on airway responses to OVA
IL-2 caused an increase in the airway responses to OVA (Fig 1 ) . The pattern of change in R L was similar in the control and IL-2–treated animals, with the exception that recovery from the early response was incomplete,
DISCUSSION
Pretreatment of BN rats with IL-2 for 4.5 days increased the early and late airway responses after OVA challenge. IL-2 enhanced airway inflammation, as evidenced by an increased cellular return from lung lavage fluid that was characterized by a higher yield of eosinophils and lymphocytes from the S/P after antigen challenge. Although IL-2 did not significantly increase the total cellular return from the tissues of the large airways, it did increase the yield of eosinophils. The mechanism of the
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Supported by the J. T. Costello Memorial Research Fund, the Research Center of the University of Montreal affiliated Hospitals, and the Medical Research Council of Canada (grant number MA 10381).
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Reprint requests: Paolo M. Renzi, MD, Meakins Christie Laboratories, 3626 St-Urbain St, Montreal, P.Q., Canada H2X 2P2.
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