Regular articleThe Flow Cytometer Model Markedly Affects Measurement of ex Vivo Whole Blood Platelet-Bound P-Selectin Expression in Patients with Chest Pain: Are We Comparing Apples with Oranges?
Section snippets
Patients
Three hundred thirty-eight consecutive patients ad- mitted to the Chest Pain Center of the emergency department of The Christ Hospital (Cincinnati, OH; n=143), St. Agnes Hospital (Baltimore, MD; n=98), and Sinai Hospital (Baltimore, MD; n=97) between December 1997 and February 1998 and meeting enrollment criteria were included in this study. Patients who were at least 21 years old with chest pain occurring at rest and lasting at least 5 minutes and with an initial onset occurring within 24
Results
Although the patterns of P-selectin changes were overall similar between the sites, platelet expression of P-selectin was consistently three times higher or greater when measured with the FACScan flow cytometer when compared to the results obtained from the EPICS XL instrument. Table 1 summarizes the data by discharge diagnosis in patients with chest pain. Individual patient data are presented at Figure 1.
Discussion
This is the first large prospective multicenter study to evaluate the diagnostic utility of two different models of flow cytometer in measuring whole blood platelet-bound P-selectin in patients with chest pain. These data demonstrate that despite similar demographics of the chest pain population evaluated, identical sampling, quality control assurance, and staining procedures between sites, the level of platelet P-selectin expression is overwhelmingly dependent on the model of flow cytometer
Acknowledgements
This study was supported in part by Centocor Diagnostics of Pennsylvania (Malvern, PA, USA). We are indebted to the staff of emergency department of the Christ Hospital (Cincinnati, Ohio, USA), St. Agnes and Sinai Hospitals (Baltimore, Maryland, USA).
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