Elsevier

Life Sciences

Volume 62, Issues 17–18, 27 March 1998, Pages 1717-1721
Life Sciences

Induction of inducible nitric oxide synthase and heme oxygenase-1 in rat glial cells

https://doi.org/10.1016/S0024-3205(98)00134-9Get rights and content

Abstract

Recent observations suggest a possible interaction between the nitric oxide (NO)/NO synthases and carbon monoxide (CO)/heme oxygenases systems. We examined the effects of lipopolysaccharide (LPS), interferon-γ (IFN-γ), and NO donor such as S-nitroso-N-acetylpenicillamine (SNAP) on induction of inducible NO synthase (iNOS) and heme oxygenase-1 (HO-1) in mixed glial cells and in rat hippocampus. In in vitro glial cells, treatment with LPS induced the expression of 130-kDa iNOS after 6 h, and NO2 accumulation and enhancement of the protein level of 33-kDa HO-1 after 12h. In addition, treatment with SNAP induced HO-1 expression after 6 h. Although a NOS inhibitor, such as NG-nitro-L-arginine (NNA), did not change LPS-induced iNOS expression, the inhibitor suppressed both NO2 accumulation and the enhancement of HO-1. Immunocytochemistry showed that LPS-treatment induced iNOS-immunoreactivity predominantly in microglia, while this treatment induced HO-1-immunoreactivity in both microglia and astrocytes. These results suggest that endogenous NO production by iNOS in microglia causes autocrine- and paracrine-induction of HO-1 protein in microglia and astrocytes in rat brain.

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