SHORT COMMUNICATIONReversal of MRP-mediated doxorubicin resistance with quinoline-based drugs
Section snippets
Materials
Iodine-125 (100.7 mCi/mL) was purchased from Amersham Biochemical Inc. LTC4 was purchased from the Cayman Chemical Co. The SCLC cells (H69 and H69/AR) were gifts from Dr. Susan P. C. Cole (Cancer Research Laboratories). The HL60 and HL60/AR cells were gifts from Dr. M. Center at Kansas State University. All other chemicals were of the highest commercial grade available.
Cell culture and plasma membrane preparation
Drug-sensitive (H69 and HL60) and -resistant (H69/AR and HL60/AR) cells were grown in RPMI 1640 medium containing 4 mM
Reversal of MRP-mediated MDR with quinoline-based drugs
Gene transfer studies have now confirmed the role of MRP in MDR in vitro13, 15. Furthermore, there is increasing evidence for the role of MRP in drug-resistant cancers 25, 27, 28, 29. However, as MRP-mediated MDR is not reversed effectively by P-gp inhibitors, there is a need to identify drugs that can reverse MRP-mediated MDR 36, 37. We have demonstrated previously direct interaction between MRP and a quinoline-based photoreactive drug (IAAQ) [44]. The photoaffinity labeling of MRP with IAAQ
Acknowledgements
The authors would like to thank Joel Karwatski for his critical reading of the manuscript. This work was supported by a grant from the Natural Science and Engineering Research Council of Canada (NSERC) to E.G. Research at the Institute of Parasitology is partially supported by the Fonds FCAR pour l’aide à la recherche.
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