NMDA receptor channels: Subunit-specific potentiation by reducing agents

doi:10.1016/0896-6273(94)90311-5

Neuron

Volume 12, Issue 5, May 1994, Pages 1031-1040

https://doi.org/10.1016/0896-6273(94)90311-5 Get rights and content

Abstract

Sulfhydryl redox agents affect NMDA receptor activity. We investigated a putative redox site in four recombinant NMDA receptors. In 293 cells expressing NR1-NR2A channels dithiothreitol (DTT) rapidly potentiated l-glutamate-activated whole-cell currents and decreased the time course of desensitization and deactivation. Part of the current potentiation (reversible component) and all kinetic changes reversed upon washout. The remaining potentiation (persistent component) was abolished by an oxidizing agent. The N-terminal 370 residues of NR2A mediate the reversible component in chimeric N R2 subunits. I n cells expressing the NR1-NR2B, -NR2C, and -NR2D channels DTT elicited only a slowly developing, persistent potentiation and increased the deactivation time course. In these, but not in NR1-NR2A, the DTT effect was rendered insensitive to reoxidation by alkylation. Reduced glutathione mimicked the DTT effects only in the NR1-NR2A receptor. Hence, molecularly distinct NMDA receptors differ profoundly in their responses to sulfhydryl redox agents.

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Neuron

NMDA receptor channels: Subunit-specific potentiation by reducing agents

Abstract

Neuron

Neuron

Neuron

Trends Pharmacol. Sci.

Neuron

Gene

J. Biol. Chem.

Neuron

FEBS Lett.

Trends Pharmacol. Sci.

Neuron

Neuron

Cell

Trends Pharmacol. Sci.

Neuron

Neuroscience

Interaction of the putative essential nutrient pyrroloquinoline quinone with the N-methyl-D-aspartate receptor redox modulatory site

J. Neurosci.

NMDA receptors and developmental plasticity in visual neocortex

A synaptic model of memory: long-term potentiation in the hippocampus

Nature

Control by asparagine residues of calcium permeability and magnesium blockade in the NMDA receptor

Science

Sulphur containing excitatory amino acids: release, activity and localization

Mol. Pharmacol.

Activation of N-methyl-D-aspartate receptors by L-glutamate in cells dissociated from adult rat hippocampus

J. Physiol.

Oxidized glutathione modulates N-methyl-D-aspartate- and depolarization-induced increases in intracellular Ca2+ in cultured rat forebrain neurons

Neurosci. Lett.

Oxidized glutathione modulates N-methyl-D-aspartate- and depolarization-induced increases in intracellular Ca²⁺ in cultured rat forebrain neurons