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Methods for determination of aldehydic lipid peroxidation products

https://doi.org/10.1016/0891-5849(89)90015-4Get rights and content

Abstract

A complex pattern of aldehydes (alkanals, 2-alkenals, 2,4-alkadienals, 4-hydroxyalkenals) is generated by peroxidizing biological samples. Several methods based on HPLC or GC-MS have been developed to qualitatively and quantitatively measure the aldehydes in tissues, cells and cell fractions exposed to various pro-oxidative stimuli. 4-Hydroxynonetal, hexanal and propanal are, besides malonaldehyde, the most abundant aldehydes formed. The high sensivity of the methods alows the measurement of physiological aldehyde levels in plasma or low density lipoproteins and this coulb be of great impotance for in two studies.

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