Research articleHepatocytes from metallothionein-I and II knock-out mice are sensitive to cadmium- and tert-butylhydroperoxide-induced cytotoxicity
References (31)
- et al.
Possible role for metallothionein in protection against radiation-induced oxidative stress: kinetics and mechanism of its reaction with Superoxide and hydroxyl radicals
Bioehim. Biophys. Acta
(1985) - et al.
Inhibition of cell membrane lipid peroxidation by cadmium-and zinc-metallothioneins
Bioehim. Biophiys. Acta
(1986) - et al.
Oxygen free radicals and metallothionein
Free Rad. Biol. Med.
(1993) - et al.
Species variation in hepatic metallothionein
Toxicol. Lett.
(1994) - et al.
Effect of several metallothionein inducers on oxidative stress defense mechanisms in rats
Toxicology
(1995) - et al.
Toxicological significance of metallothionein
Methods Enzymol.
(1991) - et al.
Copper toxicity in isolated hepatocytes
Toxicol. Appl. Pharmacol.
(1981) Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues
Anal. Biochem.
(1969)- et al.
Evaluation of the Cd/hemoglobin affinity assay for the rapid determination of metallothionein in biological tissues
Toxicol. Appl. Pharmacol.
(1982) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein binding
Anal. Biochem.
(1976)
The physiological role of zinc as an antioxidant
Free Rad. Biol. Med.
Enhanced sensitivity to oxidative stress in cultured embryonic cells from transgenic mice deficient in metallothionein I and II genes
J. Biol. Chem.
tert-Butylhydroperoxide kills cultured hepatocytes by peroxidizing membrane lipids
Arch. Biochem. Biophys.
Cytoplasmie metallothionein overexpression protects NIH 3T3 cells from teri-huty/hydroperoxide toxicity
J. Biol. Chem.
New aspects in the biological role of zinc: a stabilizer of macromolecules and biological membranes
Life Sci.
Cited by (46)
Genetics of metallothioneins in Drosophila melanogaster
2022, ChemosphereCitation Excerpt :And the location pattern in the chromosome is conservative in the Drosophila genus (Luo et al., 2020). Based on in vivo and in vitro studies on MTs of both vertebrates and invertebrates as well as plants, two major cellular functions of MT system were found: regulating the metal ion homeostasis and maintaining redox balance (Thornalley and Vašák, 1985; Lazo et al., 1995; Zheng et al., 1996; Kumari et al., 1998; Fabisiak et al., 1999; Colangelo et al., 2004; Achard-Joris et al., 2007; Jia et al., 2012; Takahashi, 2015). In D. melanogaster, the protective function of MTs against metal toxicity was elucidated in detail through studies of polymorphisms in naturally duplicated loci, knockout, and overexpression of certain MT genes.
Antioxidant Defense Mechanisms
2010, Comprehensive Toxicology, Second EditionMetallothionein-I/II null cardiomyocytes are sensitive to Fusarium mycotoxin butenolide-induced cytotoxicity and oxidative DNA damage
2010, ToxiconCitation Excerpt :In contrast, induction or overexpression of MT effectively protected against the oxidative injuries associated with streptozotocin (Cai et al., 2005), doxorubicin (Shuai et al., 2007) and ethanol (Zhou et al., 2002). Cells overexpressing MT or cells isolated from MT null mice displayed similar responses to oxidizing agents (Lazo et al., 1995; Zheng et al., 1996; Wang et al., 1999; Wang and Kang, 1999). In summary, our present study clearly shows that MT-I/II null cardiomyocytes are more sensitive than wild-type cardiomyocytes to the toxic effects of BUT, confirming basal expression of MT protects cardiomyocytes against BUT-induced cytotoxicity via inhibition of ROS production and the associated DNA damage.
Basal expression of metallothionein suppresses butenolide-induced oxidative stress in liver homogenates in vitro
2009, ToxiconCitation Excerpt :Many in vivo studies have demonstrated that MT null mice are more sensitive than wild-type mice to the toxic effects of oxidative stress-inducing agents such as acetaminophen (Liu et al., 1999b), carbon tetrachloride (Davis et al., 2001), doxorubicin (Kimura et al., 2000) and dimethylarsinic acid (Jia et al., 2004), and the mechanisms may be associated with the antioxidant activity of MT. In contrast, several studies have also proven that MT overexpression provides effective protection against the oxidative injuries associated with ethanol (Zhou et al., 2002), acetaminophen (Liu et al., 1999b), streptozotocin (Cai et al., 2005), and doxorubicin (Kimura et al., 2000; Shuai et al., 2007). Furthermore, experiments with cultured cells transfected with MT genes and cells isolated from MT transgenic or knockout mice produced similar results to oxidative stress-inducing agents (Schwarz et al., 1994; Lazo et al., 1995; Zheng et al., 1996; Wang et al., 1999; Kimura et al., 2000). Therefore, our present study is designed to reveal the antioxidant potential of basal expression of MT against butenolide-induced hepatic oxidative stress using MT-I/II null mice and the corresponding wide type mice.
Metal binding and antioxidant properties of chimeric tri- and tetra-domained metallothioneins
2008, BiochimieCitation Excerpt :The 75-aa-long CgMT1 isoform contains 21 Cys residues arranged in a typical Cys-X(1–3)-Cys motifs, whereas the 107-aa-long CgMT2 isoform displays an uncommon tri-domained α-β-β structure and contains 30 Cys residues [3,4]. MTs display in vitro antioxidant function [5–7], and in vivo experimental evidence has used cultured cells from transgenic MT-null mice deficient in mt-1 and mt-2 genes [8–10]. MT antioxidant properties may derive both from sulfhydril nucleophilicity and from metal complexation.
- 1
Present address: Cancer Center-0812, School of Medicine, University of California, San Diego, 9500 Oilman Drive, La Jolla, CA 92093-0812, USA.