A simplified timm stain procedure compatible with formaldehyde fixation and routine paraffin embedding of rat brain

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Abstract

SLOVITER, R. S. A simplified Timm stain procedure compatible with formaldehyde fixation and routine paraffin embeddingof rat brain. BRAIN RES. BULL.8(6) 771–774 1982.—A simplified sulphide/silver technique for visualizing transitional metals in rat brain is described. It consists of: (1) sulphide perfusion before formaldehyde perfusion, (2) routine paraffin embedding and sectioning, and (3) silver staining with physical developer. This method is compatible with formaldehyde fixation, eliminates the need for post-fixation in Carnoy's solution before embedding and does not require low paraffin temperatures during embedding. Once embedded, brains can be stored for months before sectioning without loss of stain quality.

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