Modulation of fast excitatory synaptic transmission by cyclothiazide and GYKI 52466 in the rat hippocampus☆
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Cited by (32)
The history of the pharmacology and cloning of ionotropic glutamate receptors and the development of idiosyncratic nomenclature
2009, NeuropharmacologyCitation Excerpt :GYKI52466 and related 2.3-benzodiazepines were used to define the majority of these postsynaptic non-NMDA receptors as AMPA receptors, rather than kainate receptors, on cortical, brainstem, thalamic and spinal neurones (Turner and Salt, 1998; Ouardouz and Durand, 1994; Világi et al., 1998; Rammes et al., 1994). In some experiments cyclothiazide and GYKI52466 had opposing effects confirming the identity of these postsynaptic receptors as the AMPA subtype (e.g. Rammes et al., 1994; Vyklicky et al., 1991). Blocking AMPA receptors with these 2,3-benzodiazepines also helped to reveal a role for kainate receptors (Paternain et al., 1995).
Climbing fiber innervation of NG2-expressing glia in the mammalian cerebellum
2005, NeuronCitation Excerpt :Indeed, NG2+ cell EPSCs were blocked by the selective AMPA receptor antagonist GYKI 53655 (50 μM; n = 5) (Figure 2B); EPSCs elicited at a holding potential of 40 mV also were blocked by the AMPA/kainate receptor antagonist NBQX (n = 31) (Figure 2C), indicating that NMDA receptors did not contribute to these responses (Wyllie et al., 1991). Cyclothiazide (CTZ), a compound that blocks desensitization of AMPA receptors and increases their apparent affinity for glutamate (Patneau et al., 1993), increased the amplitude of these responses by 99% ± 25% and slowed their decay times by 191% ± 35% (decay tau; n = 10) (Figure 2B), comparable to effects observed on neuronal EPSCs (Rammes et al., 1994; Xu-Friedman and Regehr, 2003). The rapid kinetics of NG2+ cell EPSCs and their moderate enhancement by CTZ suggests that their AMPA receptors are exposed to a high concentration of glutamate for a brief period of time, similar to the glutamate concentration transient experienced by neuronal receptors in the synaptic cleft (Clements et al., 1992; Diamond and Jahr, 1997).
Interactions between recording technique and AMPA receptor modulators
2002, Brain ResearchCitation Excerpt :Remarkably, differential effects of modulators of neural function have been reported using these two techniques. Whole cell recording studies showed that cyclothiazide increases EPSCs [18,32] in the same hippocampal synapses in which earlier studies [7,23] had found it to have little effect on field EPSPs. These observations raise the possibility that the relative contributions of rate constants to transmission are affected by recording technique.
St John's wort (Hypericum perforatum) modulates evoked potentials in guinea pig hippocampal slices via AMPA and GABA receptors
2002, European Neuropsychopharmacology
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This work was supported in part by a grant from the Deutsche Forschungsgemeinschaft (Graduiertenkolleg, SFB 353) and through a Helmholtz-stipend to W.M. from the Bundesministerium für Forschung und Technologie.
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We thank G. L. Collingridge for critically reading the manuscript and K. Clark for advicing patiently on patch clamp recording in slices.