Elsevier

Neuroscience Letters

Volume 126, Issue 1, 13 May 1991, Pages 29-32
Neuroscience Letters

Pertussis toxin-sensitive G-protein mediates galanin's inhibition of scopolamine-evoked acetylcholine release in vivo and carbachol-stimulated phosphoinositide turnover in rat ventral hippocampus

https://doi.org/10.1016/0304-3940(91)90363-XGet rights and content

Abstract

The effects of intracerebroventricular (i.c.v.) injections of pertussis toxin were investigated on the inhibitory action of galanin on acetylcholine release and phosphoinositide breakdown stimulated by muscarinic agents in rat ventral hippocampus. Pertussis toxin (0.6 μg, i.c.v., 96 h) counteracted the in vitro inhibitory effect of galanin (3.1 nmol) on phosphoinositide breakdown stimulated by carbachol without altering the stimulatory action of the cholinergic agonist on signal transduction, in miniprisms from rat ventral hippocampus. Pertussis toxin also abolished the in vivo effect of galanin on scopolamine-stimulated acetylcholine release in vivo but did not affect basal acetylcholine release. The results indicate that pertussis toxinsensitive G-protein(s) mediates the galanin receptor regulation of pre- and postsynaptic cholinergic functions in the ventral hippocampus.

Cited by (41)

  • Performance of galanin transgenic mice in the 5-choice serial reaction time attentional task

    2006, Pharmacology Biochemistry and Behavior
    Citation Excerpt :

    The inhibition of central cholinergic function by galanin is evident in studies from several different levels of analysis. On the biochemical level, galanin inhibited carbachol-stimulated PI hydrolysis (Consolo et al., 1991; Palazzi et al., 1991). In physiological studies using slice preparations of the hippocampus, galanin blocked the slow excitatory post-synaptic potential induced by acetylcholine in CA1 pyramidal neurons (Dutar et al., 1989).

  • Patterns of seizures, hippocampal injury and neurogenesis in three models of status epilepticus in galanin receptor type 1 (GalR1) knockout mice

    2004, Neuroscience
    Citation Excerpt :

    Galanin coexists with acetylcholine in septum/diagonal band complex, an area which sends cholinergic/galaninergic projections to the hippocampus (Melander et al., 1986; Consolo et al., 1994). In the hippocampus galanin inhibits both acetylcholine release (Dutar et al., 1989; Fisone et al., 1987), and postsynaptic cholinergic functions (Consolo et al., 1991). Inactivation of GalR1 in KO mice may facilitate both acetylcholine release and postsynaptic effects of pilocarpine and acetylcholine, thus promoting both components which contribute to the development of Li-pilocarpine SE.

  • Pharmacological evidence supporting a role for galanin in cognition and affect

    2001, Progress in Neuro-Psychopharmacology and Biological Psychiatry
View all citing articles on Scopus
View full text