Calcitonin gene-related peptide (CGRP) receptors are linked to cyclic adenosine monophosphate production in SK-N-MC human neuroblastoma cells
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Cited by (49)
Receptor component protein, an endogenous allosteric modulator of family B G protein coupled receptors
2020, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :For the AM1 receptor, GIPR and GLP1R, there were no significant changes to Emax or pEC50. We next investigated whether RCP knock down affected the signalling of endogenously expressed family B receptors in SK-N-MC [16], HEK293T [12] and HUVEC [17] cells. RCP knockdown by siRNA reduces agonist stimulated cAMP production by endogenously expressed CGRPR in SK-N-MC cells (Fig. 4a).
Activation of the cation channel TRPM3 in perivascular nerves induces vasodilation of resistance arteries
2019, Journal of Molecular and Cellular CardiologyCitation Excerpt :Next, we explored the mechanisms by which CGRP receptor activation in VSMC leads to vasodilation. Stimulation of the CGRP receptor has been reported to increase cyclic adenosine monophosphate (cAMP) production via the adenylate cyclase (AC) [26] and may therefore lead to protein kinase A (PKA)-mediated activation of K+ channels in VSMC and consequent hyperpolarization and arterial relaxation. To test whether activation of K+ channels is involved in the vasodilation response triggered by TRPM3 activation we compared the effects of 10 μM PS in arteries treated or not with K+ channel blockers.
Calcitonin gene-related peptide mediates an inflammatory response in Schwann cells via cAMP-dependent ERK signaling cascade
2016, Life SciencesCitation Excerpt :CGRP receptor component protein has been shown to be required for G-protein-coupled signal transduction at receptors activated by CGRP [16,31]. Although the second messenger system of CGRP receptor stimulation has been characterized quite well to involve generation of cAMP through coupled with G-protein (Gs), the distal signaling components beyond the second messenger generation are not currently well understood. [40] showed that this ERK activation by CGRP acts through Gs coupled receptors in SK-N-MC cells [40].
Localization of calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1) in human gastrointestinal tract
2012, PeptidesCitation Excerpt :Images of stained and control slides were collected and processed (colored and merged) identically. To determine if mRNA for CLR and RAMP1 were expressed in the normal human GI tract, we isolated RNA from stomach, ileum and colon biopsies/segments and from SK-N-MC cells (known to express CLR and RAMP1 [37]). Negative control without RT enzyme did not yield a PCR product, whereas, CLR and RAMP1 products were amplified from the human GI segments and SK-N-MC cells (Fig. 1).