Elsevier

Neuroscience Letters

Volume 90, Issue 3, 1 August 1988, Pages 279-284
Neuroscience Letters

Neurosteroid pregnenolone sulfate antagonizes electrophysiological responses to GABA in neurons

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Abstract

Our earlier biochemical studies suggested that the neurosteroid pregnenolone sulfate (PS) may reduce γ-aminobutyric acid (GABA) action at the Cl channel associated with GABAA receptors. In the present electrophysiological study the interaction of PS with the GABAA receptor was tested, using whole-cell voltage-clamp recordings from isolated cerebral cortical neurons of neonatal rats. At micromolar concentrations PS reversibly inhibited GABA-induced current, behaving as an allosteric receptor antagonist.

Keywords

Pregnenolone sulfate
γ-Aminobutyric acid receptor
Electrophysiology

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