Elsevier

Neuroscience Letters

Volume 59, Issue 3, 6 September 1985, Pages 291-295
Neuroscience Letters

The effect of opioids and of naloxone on Na+,K+-adenosine triphosphatase activity in frog spinal cord membrane fractions

https://doi.org/10.1016/0304-3940(85)90147-8Get rights and content

Abstract

The effects of opioids and of naloxone on ouabain-sensitive Na+,K+-adenosine triphosphatase (ATPase) activity were studied in vitro on membrane fractions from frog spinal cords. The addition of morphine and of the stable enkephalin analogue, d-Ala2,d-Leu5-enkephalin, in concentrations from 10−7 to 10−4 M ignificantly increased Na+,K+-ATPase activity. No effect was found with methionine enkephalin (Met-Enk). However, the addition of two peptidase inhibitors, captopril and phosphoramidon (10−5 M each), significantly increased Na+,K+-ATPase activity. A further increase in enzyme activity was found when Met-Enk (10−4 or 10−7 M) was added simultaneously with peptidase inhibitors. On the other hand, the addition of the opiate antagonist, naloxone, at low concentration (10−7 M) decreased the activity of Na+,K+-ATPase. These results are discussed with respect to the effect of synthetic and endogenous opioids on the activity of Na+,K+-ATPase.

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Cited by (18)

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    2003, European Journal of Pharmacology
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    Under the same experimental conditions we have shown that morphine produce a similar enhancement of mouse synaptosomal Na+,K+-ATPase activity, which is mediated by activation of μ-opioid receptors and Gi/o proteins (Masocha et al., in press). Previously other authors have also found an activation of brain and spinal cord Na+,K+-ATPase by morphine both in vitro (Jain et al., 1974; Hajek et al., 1985; Nishikawa et al., 1990) and in vivo (Desaiah and Ho, 1977; Sharma et al., 1998), and an antagonism by naloxone of this morphine effect (Desaiah et al., 1977; Nishikawa et al., 1990; Sharma et al., 1998). However, there are also studies that reported a lack of effect of morphine on Na+,K+-ATPase (Desaiah and Ho, 1977; Desaiah and Ho, 1979; Maeda et al., 1987).

  • Mechanisms involved in morphine-induced activation of synaptosomal Na<sup>+</sup>,K<sup>+</sup>-ATPase

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    Incubation in vitro of synaptosomes with morphine and buprenorphine concentration-dependently increased Na+,K+-ATPase activity. The results obtained with morphine agree with those of previous studies that also reported stimulation of Na+,K+-ATPase activity in isolated frog spinal cord [14] and rat brain cortex synaptosomes [27]. Furthermore, the in vivo administration of morphine also enhanced Na+,K+-ATPase activity in several brain areas [8,39].

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Permanent address: Institute of Hygiene and Epidemiology, 100 00 Prague 10, Czechoslovakia.

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