Up-regulation of opiate receptors by opiate antagonists in neuroblastoma-glioma cell culture: The possibility of interaction with guanosine triphosphate-binding proteins
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Cited by (30)
Sex and chronic stress alter delta opioid receptor distribution within rat hippocampal CA1 pyramidal cells following behavioral challenges
2020, Neurobiology of StressCitation Excerpt :An extensive description of previous characterization studies can be found in Mazid et al. (2016). Briefly, the DOR AB1560 has been characterized in Western blots of lysates from rat brains and in NG108-15 cells, which endogenously express DORs (Barg et al., 1984; Persson et al., 2005; Saland et al., 2005) and in preadsorption controls on tissue sections (Olive et al., 1997). Moreover, no detectable labeling of this antibody is seen in Western blots of COS-7 cells [see Supplemental Fig. 1 in (Williams et al., 2011b)], which do not endogenously express DORs (Kieffer et al., 1992) and in the dorsal raphe of DOR knockout mice [see Supplemental Fig. 1 in (Bie et al., 2010)].
Sex Differences in the Rat Hippocampal Opioid System After Oxycodone Conditioned Place Preference
2018, NeuroscienceCitation Excerpt :An extensive description of previous characterization studies can be found in Mazid et al. (2016). Briefly, the DOR AB1560 has been characterized in Western blots of lysates from rat brains and in NG108-15 cells, which endogenously express DORs (Barg et al., 1984; Persson et al., 2005; Saland et al., 2005) and in preadsorption controls on tissue sections (Olive et al., 1997). Moreover, no detectable labeling of this antibody is seen in Western blots of COS-7 cells [see Supplemental Fig. 1 in (Williams et al., 2011a)], which do not endogenously express DORs (Kieffer et al., 1992) as well as in the dorsal raphe of DOR knockout mice [see Supplemental Fig. 1 (Bie et al., 2010)].
Targeting opioid receptors with pharmacological chaperones
2014, Pharmacological ResearchCitation Excerpt :This has been shown for human as well as for rat and mouse receptors using various techniques (e.g. [22,24,55,56]). In addition, long-term opioid antagonist treatments have been found to lead to up-regulation of endogenous μOR and δOR in SH-SY5Y neuroblastoma cells and in NG108-15 neuroblastoma-glioma cells [57–60]. The ability of ORPCs to facilitate the maturation and cell surface targeting of newly synthesized receptors is an indication that the ligands act intracellularly.
Spinal interaction between the highly selective μ agonist DAMGO and several δ opioid receptor ligands in naive and morphine-tolerant mice
2013, Brain Research BulletinCitation Excerpt :The subtypes have only been concluded by pharmacological evidence while genetic basis is not unequivocal: only three main alleles are known though some research groups claim the existence of splice variants (Rossi et al., 1995). All three opioid receptors belong to the G-protein coupled receptor superfamily (GPCR) (Barg et al., 1984; Kamikubo et al., 1986; Milligan et al., 1985; Porthe et al., 1988). One of the important disadvantageous effects preventing the everyday therapeutic application is the development of tolerance to antinociceptive actions of morphine.
Ovarian hormones influence corticotropin releasing factor receptor colocalization with delta opioid receptors in CA1 pyramidal cell dendrites
2011, Experimental NeurologyCitation Excerpt :Western blot testing of the rabbit polyclonal DOR antisera from Chemicon (AB1560). No detectable labeling for the DOR was observed in COS-7 cell lysates (lanes 1–5) as these cells do not endogenously express DORs, whereas the expected 36 kDa band was observed in NG108-15 cell lysates (lanes 7-11) which have been shown to endogenously express DORs (Barg et al., 1984; Kieffer et al., 1992; Persson et al., 2005). For western blot analysis of DOR labeling specificity, COS-7 (American Type Cell Culture) and NG108-15 cells were seeded in 6-well plates at a density of 3 × 105 cells per well and allowed to grow at 37 °C for 3 days as previously described (Akama and McEwen, 2003).