Vascular adhesion molecule—1 and intercellular adhesion molecule—1 expression on rat liver cells after lipopolysaccharide administration in vivo

doi:10.1016/0270-9139(95)90162-0

Hepatology

Volume 22, Issue 5, November 1995, Pages 1538-1546

https://doi.org/10.1016/0270-9139(95)90162-0 Get rights and content

Abstract

During sepsis the infiltration of leukocytes plays a pivotal role in tissue damage. Induction of septic shock results in an early accumulation of polymorphonuclear leukocytes in the liver (after 3 hours), which is followed by an infiltration of mononuclear phagocytes (after 30 hours). Expression of adhesion molecules may contribute to the migration of leukocytes to the site of inflammation. Therefore, in the present study we determined the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) on hepatocytes, liver endothelial cells, and Kupffer cells after lipopolysaccharide (LPS) treatment of rats in vivo. Parenchymal cells showed no constitutive expression of VCAM-1 and the expression could not be upregulated by LPS treatment in vivo, whereas Kupffer and endothelial cells had a low basal expression of VCAM-1 and this expression was increased 40-fold by LPS treatment in vivo. All three cell types showed a basal expression of ICAM-1 and the expression on endothelial liver cells of untreated rats was two times higher than the expression on parenchymal and Kupffer cells. Stimulation with LPS increased the expression of ICAM-1 2.5 times for parenchymal cells and approximately 4 times for endothelial and Kupffer cells. It is concluded that the expression of adhesion molecules may contribute to the influx of leukocytes during septic shock and, therefore, play a role in tissue damage during septic shock.

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^☆: Supported by the Netherlands Organization for Scientific Research, The Hague, The Netherlands.

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Vascular adhesion molecule—1 and intercellular adhesion molecule—1 expression on rat liver cells after lipopolysaccharide administration in vivo☆

Abstract

Gastroenterology

Biochem Biophys Res Commun

J Biol Chem

Cell

Immunol Today

Cell

Lancet

Meth Cell Biol

J Biol Chem

Cell

Cell Immunol

Hepatology

J Hepatol

The pathogenesis of sepsis

Ann Intern Med

Manifestations of sepsis

Arch Intern Med

Pathogenic mechanisms of septic shock

N Engl J Med

Time course of cellular distribution of endotoxin in liver, lung and kidney of rats

Br J Exp Pathol

Clearance, tissue distribution, and cellular localization of iv injected LPS in rabbits

J Immunol

Uptake by liver cells of endotoxin following its intravenous injection

Lab Invest

Production of hepatocyte stimulating factor of rat Kupffer cells induced by lipopolysaccharide: partial characterization and effects on alpha 2 macroglobulin gene expression in cultured adult rat hepatocytes

J Clin Lab Immunol

In vivo biologic and immunohistochemical analysis of interleukin-1 alpha, beta and tumor necrosis factor during experimental endotoxemia

Am J Pathol