Dopamine-dependent cyclic AMP generating system in chick retina and its relation to melatonin biosynthesis

https://doi.org/10.1016/0197-0186(95)80012-AGet rights and content

Abstract

Stimulation of a D4-like dopamine (DA) receptors inhibits a cAMP-dependent increase in serotonin N-acetyltransferase activity and melatonin biosynthesis in the chick retina. In order to gain more insight into the molecular mechanisms underlying this suppressive action of DA, the effects of selective stimulation of the D2-family of DA receptors (including the D4-subtype) on cAMP formation were examined in chick retina using two experimental approaches: measurements of adenylyl cyclase activity in retinal homogenates, and cAMP accumulation in eye cup preparations prelabeled with [3H]adenine. The DA-sensitive adenylyl cyclase system is well expressed in chick retina. DA increased both basal and forskolin-stimulated adenylyl cyclase activity. This effect of DA was antagonized by SCH 23390 (a blocker of D1-family of DA receptors) and not affected by sulpiride (a D2-family blocker). Incubation of retinal homogenates with quinpirole (a predominant agonist of D3/D4 DA receptor subtypes) did not produce any major changes in adenylyl cyclase activity. On the other hand, activation of D4-like DA receptor subtype by quinpirole decreased forskolin-stimulated cAMP formation in intact chick retinas maintained in “eye-cup” preparations. It is suggested that D4-like DA receptors regulating melatonin biosynthesis in chick retina may be indirectly linked to the cAMP generating system.

References (58)

  • J.-C. Schwartz et al.

    The dopamine receptor family: molecular biology and pharmacology

    The Neurosciences

    (1992)
  • J.C. Stoof et al.

    Growth hormone producing cells in Lymnaea stagnalis as a model system for mammalian dopamine receptors?

    Trends Pharmac. Sci.

    (1986)
  • L. Vallar et al.

    Mechanisms of signal transduction at the dopamine D2 receptor

    Trends Pharmac. Sci.

    (1989)
  • A.F. Wiechmann et al.

    Localization of mRNA encoding the indoleamine synthesizing enzyme, hydroxyindole-O-methyltransferase, in chicken pineal gland and retina by in situ hybridization

    Neurosci. Lett.

    (1993)
  • P. Witkovsky et al.

    Functional roles of dopamine in the vertebrate retina

    Prog. Retinal Res.

    (1991)
  • J.B. Zawilska et al.

    Regulatory mechanisms in melatonin biosynthesis in retina

    Neurochem. Int.

    (1992)
  • J.B. Zawilska et al.

    Dopamine receptor regulating serotonin N-acetyltransferase activity in chick retina represents a D4-like subtype: pharmacological characterization

    Neurochem. Int.

    (1994)
  • J.B. Zawilska et al.

    Does D4 dopamine receptor mediate the inhibitory effect of light on melatonin biosynthesis in chick retina?

    Neurosci. Lett.

    (1994)
  • J.H. Brown et al.

    Stimulation by dopamine of adenylate cyclase in retinal homogenates and of adenosine-3′:5′-cyclic monophosphate formation in intact retina

    Proc. natn. Acad. Sci. U.S.A.

    (1972)
  • B.M. Brown et al.

    A simple and sensitive saturation assay method for the measurement of adenosine 3′:5′-monophosphate

    Biochem. J.

    (1971)
  • M.A. Castellano et al.

    Transfected D2 short dopamine receptors inhibit voltage-dependent potassium current in neuroblastoma × glioma hybrid (NG108-15) cells

    Molec. Pharmac.

    (1993)
  • L. Castelletti et al.

    Potassium channels involved in the transduction mechanism of dopamine D2 receptors in rat lactotrophs

    J. Physiol., Lond.

    (1989)
  • C. Chabert et al.

    Characterization of the functional activity of dopamine ligands at human recombinant dopamine D4 receptors

    J. Neurochem.

    (1994)
  • A.I. Cohen et al.

    Dopamine and its agonists reduce a light-sensitive pool of cyclic AMP in mouse photoreceptors

    Vis. Neurosci.

    (1990)
  • A.I. Cohen et al.

    Photoreceptors of mouse retinas possess D4 receptors coupled to adenylate cyclase

    Proc. natn. Acad. Sci. U.S.A.

    (1992)
  • A. Dearry et al.

    D2 dopamine receptors in human retina: cloning of cDNA and localization of mRNA

    Cell. Molec. Neurobiol.

    (1991)
  • T. Deguchi

    Role of adenosine 3′,5′-monophosphate in the regulation of circadian oscillator of serotonin N-acetyltransferase activity in cultured chick pineal glands

    J. Neurochem.

    (1979)
  • M.C.F. De Mello et al.

    Regulation of dopamine- and adenosine-dependent adenylate cyclase systems of chicken embryo retina cells in culture

    Proc. natn. Acad. Sci. U.S.A.

    (1982)
  • J.E. Freedman et al.

    Single K+ channels activated by D2 dopamine receptors in acutely dissociated neurons from rat corpus striatum

    Proc. natn. Acad. Sci. U.S.A.

    (1988)
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    Part of these data were presented at the International Symposium “Eye-Pineal Relationships”, held in Łódź, Poland (21–25 September 1994) and organized by J.Z. Nowak and R.J. Reiter. J.Z. Nowak acted as executive editor in the refereeing of this work.

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