Effects of vitamin B6 and its related compounds on survival of cultured brain neurons

doi:10.1016/0168-0102(96)81279-X

Neuroscience Research

Volume 24, Issue 1, December 1995, Pages 61-65

https://doi.org/10.1016/0168-0102(96)81279-X Get rights and content

Abstract

The effects of pyridoxine and its derived cofacter, pyridoxal phosphate (PLP) on the survival of primary cultured neurons from fetal rat brain were investigated. Pyridoxine and PLP significantly promoted the neuronal survival of various brain regions in high cell density culture (10⁵ cells/cm²), but showed no positive effects on hippocampal neurons in low cell density culture (5 × 10³ cells/cm²). This neurotrophic effect of PLP was remarkably suppressed by picrotoxin and ifenprodil. Aminooxyacetic acid (AOAA), an inhibitor of PLP dependent enzymes, caused significant neuronal loss by itself, and largely counteracted the neurotrophic effect of PLP. Taken together, we presume that vitamin B₆ afforded the survival-promoting activities of cultured neurons by virtue of its crucial coenzymatic actions in the biosynthesis of putative neurotransmitters.

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Research reportEffects of vitamin B6 and its related compounds on survival of cultured brain neurons

Abstract

Brain Res.

Neuroscience

Neurosci. Lett.

Brain Res.

Brain Res.

Biochem. Pharmacol.

Exp. Neurol.

Neuroscience

Aminooxyacetic acid results in excitotoxic lesions by a novel indirect mechanism

J. Neurochem.

Influence of the malate-aspartate shuttle on oxidative metabolism in synaptosomes

J. Neurochem.

Research report
Effects of vitamin B₆ and its related compounds on survival of cultured brain neurons