Cell
Volume 51, Issue 4, 20 November 1987, Pages 569-577
Journal home page for Cell

Article
Colocalization of calcium-dependent protease II and one of its substrates at sites of cell adhesion

https://doi.org/10.1016/0092-8674(87)90126-7Get rights and content

Abstract

Adhesion plaques, specialized regions of the plasma membrane where a cell contacts its substratum, are dynamic structures. However, little is known about how the protein-protein interactions that occur at adhesion plaques are controlled. One mechanism by which a cell might modulate its associations with the substratum is by selective, regulated proteolysis of an adhesion plaque component. Here we show that the catalytic subunit of the calcium-dependent protease type II (CDP-II) is localized in adhesion plaques of several cell types (BS-C-1, EBTr, and MDBK). We have compared the susceptibility of the adhesion plaque constituents vinculin, talin, and α-actinin to calcium-dependent proteolysis in vitro and have found talin to be the preferred substrate for CDP-II. The colocalization of a calcium-requiring proteolytic enzyme and talin in adhesion plaques raises the possibility that calcium-dependent proteolytic activity provides a mechanism for regulating some aspect of adhesion plaque physiology and function via cleavage of talin.

References (58)

  • J.R. Feramisco et al.

    A rapid purification of α-actinin, filamin, and a 130,000-dalton protein from smooth muscle

    J. Biol. Chem.

    (1980)
  • J.E.B. Fox et al.

    Calcium-dependent proteolysis occurs during platelet aggregation

    J. Biol. Chem.

    (1983)
  • J.E.B. Fox et al.

    Identification of two proteins (actin-binding protein and P235) that are hydrolyzed by endogenous Ca+2-dependent protease during platelet aggregation

    J. Biol. Chem.

    (1985)
  • Y. Gache et al.

    Susceptibility of platelet α-actinin to a calcium-activated neutral protease

    Biochim. Biophys. Res. Commun.

    (1984)
  • A. Kitahara et al.

    Selective localization of calpain I (the low Ca2+ requiring form of Ca2+-dependent cysteine proteinase) in B cells of human pancreatic islets

    FEBS Lett.

    (1985)
  • R.L. Mellgren et al.

    The protein inhibitor of calcium-dependent proteases: purification from bovine heart and possible mechanisms of regulation

    Arch. Biochem. Biophys.

    (1983)
  • T. Murachi

    Calpains and calpastatin

    Trends Biochem. Sci.

    (1983)
  • T. O'Halloran et al.

    Purification of a 190 kDa protein from smooth muscle: relationship to talin

    Biochim, Biophys. Acta

    (1986)
  • M. Sakon et al.

    Two forms of Ca++-activated neutral protease in platelets

    Thromb. Res.

    (1981)
  • J.E. Schollmeyer

    Role of Ca2+ and Ca2+-activated protease in myoblast fusion

    Exp. Cell Res.

    (1986)
  • R. Sealock et al.

    Talin is a postsynaptic component of the rat neuromuscular junction

    Exp. Cell Res.

    (1986)
  • B.M. Sefton et al.

    Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus

    Cell

    (1981)
  • I.I. Singer

    The fibronexus: a transmembrane association of fibronectin-containing fibers and bundles of 5 nm microfilaments in hamster and human fibroblasts

    Cell

    (1979)
  • G.G. White

    Calcium-dependent protease in platelets

    Response of calcium-activated protease in normal and thrombasthenic platelets to aggregating agents

    Biochim. Biophys. Acta

    (1980)
  • K.M. Yamada et al.

    Cell surface protein decreases microvilli and ruffles on transformed mouse and chick cells

    Cell

    (1976)
  • N. Yoshida et al.

    Purification of human platelet calcium-activated neutral protease

    J. Biol. Chem.

    (1983)
  • N. Yoshimura et al.

    Intracellular localization of two distinct calcium proteases (calpain I and calpain II) as demonstrated using discriminative antibodies

    J. Biol. Chem.

    (1984)
  • S.K. Akiyama et al.

    Characterization of a 150-kD avian cell surface antigen as a fibronectin-binding molecule

    J. Cell Biol.

    (1986)
  • M.C. Beckerle

    Identification of a new protein localized at sites of cell-substrate adhesion

    J. Cell Biol.

    (1986)
  • Cited by (0)

    View full text