Rapid extraction of oxygenated metabolites of arachidonic acid from biological samples using octadecylsilyl silica

https://doi.org/10.1016/0090-6980(80)90144-6Get rights and content

Abstract

A rapid procedure for the efficient extraction of prostaglandins, thromboxanes and hydroxy fatty acids from urine, plasma and tissue homogenates has been developed. Fractions containing these substances are acidified and passed through a column of octadecylsilyl silica, which retains oxygenated metabolites of arachidonic acid. Phospholipids, proteins and very polar materials either are not retained or can be eluted with dilute aqueous ethanol. Nonpolar lipids and monohydroxy fatty acids are then eluted with petroleum ether or benzene. Subsequent elution of the column with methyl formate gives a fraction containing prostaglandins and thromboxanes which is much less contaminated with extraneous material than that obtained by conventional extraction of aqueous media with organic solvents. The methyl formate can be removed rapidly under a stream of nitrogen and the components of the sample purified directly by high pressure liquid chromatography (HPLC). An improved method for the purification of prostaglandins and TXB2 by HPLC on silica columns is reported.

References (8)

There are more references available in the full text version of this article.

Cited by (548)

  • Multiplex quantitative analysis of eicosanoid mediators in human plasma and serum: Possible introduction into clinical testing

    2017, Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
  • In vivo α-hydroxylation of a 2-alkylindole antagonist of the OXE receptor for the eosinophil chemoattractant 5-oxo-6,8,11,14-eicosatetraenoic acid in monkeys

    2017, Biochemical Pharmacology
    Citation Excerpt :

    After storing overnight at −80 °C the samples were thawed and centrifuged. The concentration of MeOH was adjusted to 30% by the addition of water and each sample was loaded onto a Sep-Pak C18 cartridge (Waters Corporation, Milford Massachusetts) [12], which was washed with 30% MeOH prior to the elution of indole metabolites with 100% MeOH. After evaporation of the solvent using a rotary evaporator the residue was dissolved in 30% MeOH and analyzed by precolumn extraction/RP-HPLC [13].

View all citing articles on Scopus
View full text