Localization of 5-HT1B, 5-HT1Dα, 5-HT1E and 5-HT1F receptor messenger RNA in rodent and primate brain

doi:10.1016/0028-3908(94)90067-1

Neuropharmacology

Volume 33, Issues 3–4, March–April 1994, Pages 367-386

https://doi.org/10.1016/0028-3908(94)90067-1 Get rights and content

Abstract

In situ hybridization histochemistry (ISHH) was used to study the distribution of various 5-HT₁ receptor messenger RNAs (mRNA) in the mammalian nervous system. Since the cDNAs encoding the different 5-HT₁ receptors, have not been cloned in one single species, brains of the species appropriate for the 5-HT₁ receptor messenger RNA (mRNA) have been used. Thus, 5-HT_1B and 5-HT_1Dα, mRNA were determined in rat and mouse brain, while 5-HT_1E and 5-HT_1F mRNA were studied in human (and monkey) and guinea-pig brain, respectively.

5-HT_1B and 5-HT_1Dα hybridization signals were predominantly present in caudate-putamen and cortical areas; in addition, 5-HT_1B mRNA was also detected in hippocampus, cerebellum and cerebral arteries. In general, the distribution of 5-HT_1B mRNA was characterized by high densities, whereas 5-HT_1Dα mRNA was expressed at very low levels. Comparison of the localization of the mRNAs to the regional distributions of the 5-HT_1B and 5-HT_1D binding sites in rat brain (described in a previous study), revealed that both receptor subtypes could be putative presynaptic heteroreceptors, modulating the release of various neurotransmitters in the central nervous system.

The mRNA encoding the recently cloned 5-HT_1E receptor, which has low affinity for the 5-HT₁ receptor ligand 5-carboxamidotryptamine (5-CT), was localized in human brain. It was found to be present in cortical areas, caudate, putamen and amygdala, areas known to contain 5-CT insensitive 5-HT₁ binding sites.

The regional distribution of the 5-HT_1Fip mRNA was determined in guinea-pig brain: high densities were observed in various cortical areas, the hippocampal formation and claustrum, which are regions known to contain 5-CT insensitive 5-HT₁ or non 5-HT_1A/1B/1C/1D[³H]5-HT binding sites.

Altogether, this ISHH study describes the distribution of mRNAs of recently cloned 5-HT₁ receptors in rodent and primate brain and compares these results to the distribution of the heterogeneous population of 5-HT₁ binding sites.

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^*: Present address: Wyeth Research (UK) Ltd, Taplow, Maidenhead, Berks SL6 OPH, U.K.

^†: Present address: Neurology Service, Massachusetts General Hospital, 32 Fruit Street, Boston, MA 02114, U.S.A.

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Localization of 5-HT1B, 5-HT1Dα, 5-HT1E and 5-HT1F receptor messenger RNA in rodent and primate brain

Abstract

Eur. J. Pharmac.

Eur. J. Pharmac.

Neuroscience

Molec. Brain Res.

Eur. J. Pharmac.

Biochem. biophys. Res. Commun.

Trends Pharmac. Sci.

Trends Pharmac. Sci.

Eur. J. Pharmac.

Trends Pharmac. Sci.

Eur. J. Pharmac.

J. biol. Chem.

FEES Lett.

Eur. J. Pharmac.

Eur. J. Pharmac.

Neurosci. Lett.

Eur. J. Pharmac.

Brain Res.

Brain Res.

Brain Res.

Neuroscience

Neuroscience

Brain Res.

Brain Res.

Neyurochem. Int.

The rat 5-hydroxytryptamineie1B receptor is the species homologue of the human 5-hydroxytryp-tamine1Dβ receptor

Molec. Pharmac.

Cloning of another human serotonin (5-HT1F): a fifth 5-HT1 receptor subtype coupled to the inhibition of adenylate cyclase

Isolation of a mouse “5-HTlE-like” serotonin receptor expressed predominantly in the hippocampus

J. biol. Chem.

A new member of the serotonin receptor family: structure and functional expression of a 5-HT1D like receptor

J. Rec. Res.

Evidence that 5-HT agonist-induced rotational behaviour in the rat is mediated via 5-HT1 receptors

Psychopharmacology

Biochemical and pharmacological characterization of serotonin-O-carboxymethylglycyl[125I]-iodotyrosinamide, a new radioiodinated probe for 5-HT1B and 5-HT1D binding sites

J. Neurochem.

Localization of 5-HT_1B, 5-HT_1Dα, 5-HT_1E and 5-HT_1F receptor messenger RNA in rodent and primate brain

The rat 5-hydroxytryptamineie_1B receptor is the species homologue of the human 5-hydroxytryp-tamine_1Dβ receptor

Cloning of another human serotonin (5-HT_1F): a fifth 5-HT₁ receptor subtype coupled to the inhibition of adenylate cyclase

Evidence that 5-HT agonist-induced rotational behaviour in the rat is mediated via 5-HT₁ receptors

Biochemical and pharmacological characterization of serotonin-O-carboxymethylglycyl[¹²⁵I]-iodotyrosinamide, a new radioiodinated probe for 5-HT_1B and 5-HT_1D binding sites