Endorphinergic modulation of immune function: Potent action of the dipeptide glycyl-L-glutamine
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2021, International ImmunopharmacologyCitation Excerpt :The Lymphocyte viability of blood, spleen and thymus was measured by the trypan blue exclusion method using Neubauer's hemocytometer under a bright-field microscope as described by Bandyopadhyay and Poddar [28]. The standard microculture technique described by McCain et al. [29] was used to assess the lymphocytic mitogen (Con A)-induced proliferating activity of blood, spleen, and thymus. As per the method, the blood, spleen, or thymus lymphocytic cells were cultured in triplicate and then resuspended in CM separately.
Glycyl-L-glutamine attenuates NPY-induced hyperphagia via the melanocortin system
2020, Neuroscience LettersCitation Excerpt :These facts suggest that Gly-Gln may be a counter-regulated neurotransmitter via hypothalamus. Importantly, it was reported that Gly-Gln has a bidirectional effect in a dose-dependent manner, in vitro, in the context of immune cells [18]. These results suggest that Gly-Gln is a physiological neuromodulator with a negative feedback effect on NPY-induced hyperphagia.
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2010, Agricultural Sciences in China