Comparison between skin-sensitizing antibody titers and leukocyte sensitivity measurements as an index of the severity of ragweed hay fever☆
References (18)
- et al.
Skin-sensitizing Antibody Titer. III. Relationship of the Skin-Sensitizing Antibody Titer to the Intracutaneous Skin Test to the Tolerance of Injections of Antigens and to the Effects of Prolonged Treatment With Antigen
J. Allergy
(1964) - et al.
Skin-Sensitizing Antibody Titer. I. Relationship of Skin-Sensitizing Antibody Titer to the Occurrence of Symptoms in Untreated Persons With a Positive Skin Test
J. Allergy
(1963) - et al.
In Vitro Assay of Allergenic Properties of Ragweed Pollen Antigens
J. Allergy
(1966) - et al.
Histamine Release From Leukocytes of Hypersensitive Individuals. II. Reduced Sensitivity of Leukocytes After Injection Therapy
J. Allergy
(1967) Clinical Immunology and Allergy
- et al.
Allergy in Pediatric Practice
- et al.
In Vitro Studies of Human Ragweed Allergy: Changes in Cellular and Humoral Activity Associated With Specific Desensitization
J. Clin. Invest
(1966) - et al.
Leukocyte-Sensitizing Activity in the Sera of Ragweed Hay Fever Patients: Seasonal Changes Demonstrated by In Vitro Measurements
J. Allergy
(1967) - et al.
Studies on the Mechanisms of Hypersensitivity Phenomena. IX. Histamine Release From Human Leukocytes by Ragweed Pollen Antigen
J. Exper. Med
(1964)
Cited by (29)
Cellular immune response parameters that influence IgE sensitization
2012, Journal of Immunological MethodsCitation Excerpt :The release of histamine from leukocyte suspensions challenged with antigen has long been used as an in vitro marker for immediate hypersensitivity. Decades ago, the percent of histamine released from the basophil in response to allergen (relative to total histamine content) was noted to correlate strongly with the severity of clinical symptoms experienced by individuals allergic to these allergens (Lichtenstein et al., 1967, 1968). For this reason, in vitro basophil histamine release has been used as a sensitive indicator of an individual's allergic status, with the caveat that false positive results do occur and that a positive result is only meaningful to diagnose disease in the context of a supporting clinical history.
Basophil models of homeopathy: a sceptical view
2010, HomeopathyCitation Excerpt :In laboratory studies, cross-linking is usually achieved by the means of anti-IgE although in studies investigating allergic responses the allergen is employed. Basophil activation has been studied in a number of different ways: changes in basophil staining with alcian blue,9 measurement of histamine release10 and flow cytometric methods to determine changes in basophil surface markers after activation.11,12 The alcian blue staining method as described by Gilbert and Ornstein in 1975 provided a rapid method for the counting of basophils.9
Legends in allergy: Philip S. Norman and Lawrence M. Lichtenstein-The Hopkins Experience
2007, Journal of Allergy and Clinical ImmunologyCitation Excerpt :That same year, the group and Hill15 also expounded on the effects of ragweed immunotherapy on these same parameters and made the following hallmark observations: (1) antiragweed IgE antibodies rise in untreated patients as they are naturally exposed to ragweed pollen during the season; (2) in immunized patients, treatment induces an initial rise in IgE antibodies, which over time declines and is not boosted with subsequent seasonal exposure; (3) the treatment-induced increase in IgG blocking antibody correlates with the observed decrease in serum IgE antibody; (4) IgE antibody on basophils appears to correlate with the untreated patients' serum IgE; and (5) in untreated patients, symptom scores strongly correlate with both their serum IgE and the sensitivity of their basophils to Ag E–induced histamine release. The earlier studies cited on cell sensitivity11,12 combined with the published results demonstrating the ability to measure antibody response after allergen immunotherapy10,11,15,16 set the stage for a series of definitive studies of immunotherapy with ragweed allergen published by the Hopkins group from 1968 to 1972.17-20 In 1963, Lowell and Franklin21 reported that in 24 patients with ragweed allergy and seasonal fall hay fever symptoms, a significant reduction in symptoms was noted during the peak of the ragweed season by the patients on active therapy compared with the placebo group (P = .05).
In vitro assays for the diagnosis of IgE-mediated disorders
2004, Journal of Allergy and Clinical ImmunologyCitation Excerpt :Before reputable manufacturers release these allergosorbents, however, each is generally validated with at least several characterized human sera that are known to contain relevant allergen-specific IgE antibody. Activation of the secretory response in human basophils in the presence of IgE cross-linking doses of allergen has been extensively used by research laboratories as an alternative measure of allergen-specific IgE antibody.43,44 However, to date, these assays have not been widely used in the diagnosis of allergic patients for a number of reasons.
Systemic immunologic and inflammatory aspects of allergic rhinitis
2000, Journal of Allergy and Clinical ImmunologyRelationship between the early, late, and rechallenge reaction to nasal challenge with antigen: Observations on the role of inflammatory mediators and cells
1990, The Journal of Allergy and Clinical Immunology
- ☆
Supported by Grants AI 07290, AI 04866, and AI 05780 from the National Institute of Allergy and Infectious Diseases.
- ∗
American College of Physicians Research and Teaching Scholar.
- ∗∗
Recipient of a research career development award from the National Institute of Allergy and Infectious Diseases.