The establishment of a line (WISH) of human amnion cells in continuous cultivation

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Abstract

  • 1.

    1. A description is given of the events occurring in the alteration of a culture of primary human amnion cells to an established cell line (WISH). Photographic documentation of the event is presented.

  • 2.

    2. Characteristics of the cell line are given with respect to virus spectrum, preservation at −70 °C, and growth in suspended culture.

  • 3.

    3. Evidence is presented which suggests that the initial alteration occurred in vitro and not from a pre-existing altered cell in the amnion.

  • 4.

    4. A comparison is made between the events observed in the establishment of this cell line (WISH) and those described by other workers who have observed this phenomenon.

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This work was supported by a grant (C-4534) from the National Institutes of Health, United States Public Health Service.

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