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Anti-IL-5 monoclonal antibody inhibits allergic late phase bronchial eosinophilia in guinea pigs: a therapeutic approach

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Abstract

In this study the effect of purified rat anti-mouse IL-5 monoclonal antibody on aeroallergen-induced infiltration of eosinophils in the bronchoalveolar lavage fluid of guinea pigs was studied. The i.p. injection of anti-IL-5 antibody 4 h after aeroallergen challenge inhibited eosinophil infiltration in a dose-dependent fashion. The resulting ED50 was 10 (3.4–32.8) μg/kg. The clinical therapeutic usefulness of anti-IL-5 or anti-IL-5-producing cells in asthma/allergy treatment remains to be an intriguing possibility.

References (12)

  • A.R. Gulbenkian et al.

    IL-5 modulates eosinophil accumulation in allergic guinea pig lung

    J. Allergy Clin. Immunol.

    (1991)
  • P. Andersson

    Antigen-induced bronchial anaphylaxis in actively sensitized guinea pigs. Patterns of response in relation to immunization regimen

    Allergy

    (1980)
  • N. Chand et al.

    Inhibition of Aeroallergen-Induced Immediate Asthmatic Response and Late Phase Bronchoalveolar Eosinophilia by Azelastine in Actively Sensitized Guinea Pigs

    Am. Rev. Respir. Dis.

    (1990)
  • N. Chand et al.

    Therapeutic approach for the evaluation of antiasthma compounds in allergic bronchial inflammation in guinea pigs

    Am. Rev. Respir. Dis.

    (1991)
  • C.J. Corrigan et al.

    CD4 T-lymphocyte activation in acute severe asthma - relationship to disease severity and atopic status

    Am. Rev. Respir. Dis.

    (1990)
  • R.T. Costello

    A Unopette for eosinophil counts

    Am. J. Clin. Pathol.

    (1970)
There are more references available in the full text version of this article.

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