Mass changes of inositol(1,4,5) trisphosphate in trachealis muscle following agonist stimulation

doi:10.1016/0014-2999(89)90269-0

European Journal of Pharmacology

Volume 164, Issue 3, 30 May 1989, Pages 587-590

https://doi.org/10.1016/0014-2999(89)90269-0 Get rights and content

Abstract

Agonist-stimulated changes in D-Ins(1,4,5)P₃ has been examined in tracheal smooth muscle using a specific radioreceptor assay utilizing the binding of [³H] or [³²P]Ins(1,4,5)P₃ to a bovine adrenal cortex preparation. Carbachol produced a maximal increase in Ins(1,4,5)P₃ concentration after 5 s (basal: 12.9 ± 0.8; stimulated: 27 ± 1.5 pmol/mg protein) with an EC₅₀ of 6.7 ± 0.8 μM. With longer stimulation periods Ins(1,4,5)P₃ rapidly returned to basal values by 30 s. This is first report of mass measurements of Ins(1,4,5)P₃ in smooth muscle.

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Short communicationMass changes of inositol(1,4,5) trisphosphate in trachealis muscle following agonist stimulation

Abstract

J. Biol. Chem.

Biochem. Biophys. Res. Commun.

Biochem. Biophys. Res. Commun.

European J. Pharmacol.

J. Biol. Chem.

Short communication
Mass changes of inositol(1,4,5) trisphosphate in trachealis muscle following agonist stimulation