The maintenance of cytochrome P-450 in rat hepatocyte culture: Some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P-450 system

doi:10.1016/0009-2797(90)90055-R

Chemico-Biological Interactions

Volume 74, Issues 1–2, 1990, Pages 1-31

https://doi.org/10.1016/0009-2797(90)90055-R Get rights and content

Abstract

Treatments affecting the loss of cytochrome P-450 in rat hepatocyte culture are reviewed and the way in which these have produced an understanding of the mechanisms involved are discussed extensively. A simple way to prevent the loss of P-450 in hepatocytes is to culture them with 0.5 mM metyrapone which appears to restore the cytochromes' synthesis and degradation to steady state values. Knowledge of this mechanism has led to the formulation of special culture medium and the application of both culture systems to the study of drug metabolism and toxicity are described. Finally the effect of these culture systems on the expression of the multiple forms of cytochrome P-450 are presented to illustrate the potential of cultured hepatocytes in induction studies.

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Review articleThe maintenance of cytochrome P-450 in rat hepatocyte culture: Some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P-450 system

Abstract

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Biochem. Pharmacol.

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Biochem. Biophys. Res. Commun.

Biochem. Pharmacol.

Febs Lett.

Arch. Biochem. Biophys.

Biochim. Biophys. Acta

Biochem. Pharmacol.

Toxicology

Biochem. Pharmacol.

Exp. Cell. Res.

J. Biol. Chem.

J. Biol. Chem.

Drug metabolism and effects of carcinogens in cultured hepatic parenchymal cells

Pharmacol. Rev.

Factors regulating drug metabolism in intact hepatocytes

Pharmacol. Rev.

Phenotypic stability of adult rat hepatocytes in primary monolayer culture

Ann. N.Y. Acad. Sci.

Biochemical functions of adult rat hepatocytes in priamry culture

Ann. N.Y. Acad. Sci.

Relationship between the ability of nicotinamide to maintain nicotinamide-adenine dinucleotide in rat liver cell culture and its effect on cytochrome P-450

Biochem. J.

Multiplicity of mamalian microsomal cytochrome P-450

Pharmacol. Rev.

Primary cultures and the levels of cytochrome P-450 in hepatocytes from mouse, rat, hamster and rabbit liver

In vitro

Isolation, culture and transplantation of human hepatocytes

J. Natl. Cancer Inst.

Hepatic cytochrome P-450

Essays Biochem.

The regulation of rat liver tryptophan pyrolase by its cofactor haem

Biochem. J.

Lactic dehydrogenase activity in blood

Review article
The maintenance of cytochrome P-450 in rat hepatocyte culture: Some applications of liver cell cultures to the study of drug metabolism, toxicity and the induction of the P-450 system