Spermidine and cardiovascular control in nucleus tractus solitarii in rat

doi:10.1016/0006-8993(93)90272-O

Brain Research

Volume 620, Issue 1, 20 August 1993, Pages 72-77

https://doi.org/10.1016/0006-8993(93)90272-O Get rights and content

Abstract

Cardiovascular effects elicited by unilateral microinjection of polyamines into the nucleus tractus solitarii (NTS) were tested in anesthetized rats. Spermidine (50–900 pmol) produced dose-dependent depressor and bradycardiac responses that were similar to those elicited byN-methyl-D-aspartate (NMDA; 0.1–1.2 pmol). The polyamines, putrescine and spermine, did not produce significant cardiovascular responses. The cardiovascular responses induced by spermidine and NMDA were almost eliminated by prior injection of the noncompetitive NMDA antagonist MK801 into NTS. On the other hand, a competitive antagonist 2-amino-5-phosphonovaleric acid (APV) blocked responses to NMDA but only attenuated those to spermidine. These findings support an action of the polyamine spermidine at the NMDA receptor complex in NTS where it could participate in modulating cardiovascular activity.

References (32)

FaggG.E.
Phencyclidine and related drugs bind to the activatedN-methyl-d-aspartate receptor-channel complex in rat brain membranes
Neurosci. Lett.
(1987)
GiladG.M. et al.
Polyamine uptake, binding and release in rat brain
Eur. J. Pharmacol.
(1991)
GordonF.J. et al.
Non-NMDA receptors in the nucleus of the tractus solitarius play the predominant role in mediating aortic baroreceptor reflexes
Brain Res.
(1991)
HarikS.I. et al.
Putrescine: regional distribution in the nervous system of the rat and the cat
Brain Res.
(1974)
IqbalZ. et al.
Polyamines appear to be second messengers in mediating Ca²⁺ fluxes and neurotransmitter release in potassium-depolarized synaptosomes
Biochem. Biophys. Res. Commun.
(1985)
KuboT. et al.
Evidence ofN-methyl-d-aspartate receptor-mediated modulation of the aortic baroreceptor reflex in the rat nucleus tractus solitarii
Neurosci. Lett.
(1988)
MaciverC.R. et al.
Opposite effects of spermine and arcaine on responses ofN-methyl-d-aspartate receptors expressed inXenopus oocytes
Neurosci. Lett.
(1991)
PullanL.M. et al.
Spermine reciprocally changes the affinity of NMDA receptor agonists and antagonists
Eur. J. Pharmacol. Mol. Pharmacol.
(1991)
SeilerN.
Polyamine metabolism and function in brain
Neurochem. Int.
(1981)
ShawG.G.
The polyamines in the central nervous system
Biochem. Pharmacol.
(1979)

SlotkinT.A. et al.

Role of ornithine decarboxylase and the polyamines in nervous system development: a review

Brain Res. Bull.

(1986)

TalmanW.T.

Kynurenic acid microinjected into the nucleus tractus solitarius of rat blocks the arterial baroreflex but not responses to glutamate

Neurosci. Lett.

(1989)

TalmanW.T. et al.

Antagonism of the barorecetor reflex by glutamate diethyl ester, an antagonist tol-glutamate

Brain Res.

(1981)

WatkinsJ.C. et al.

Structure-activity relationships in the development of excitatory amino acid receptor agonists and competitive antagonists

Trends Pharmacol. Sci.

(1990)

WilliamsK. et al.

Modulation of the NMDA receptor by polyamines

Life Sci.

(1991)

YonedaY. et al.

Identification and characterization of specific binding sites of [³H]spermidine in synaptic membranes of rat brain

Brain Res.

(1991)

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Spermidine and cardiovascular control in nucleus tractus solitarii in rat

Abstract

Neurosci. Lett.

Eur. J. Pharmacol.

Brain Res.

Brain Res.

Biochem. Biophys. Res. Commun.

Neurosci. Lett.

Neurosci. Lett.

Eur. J. Pharmacol. Mol. Pharmacol.

Neurochem. Int.

Biochem. Pharmacol.

Brain Res. Bull.

Neurosci. Lett.

Brain Res.

Trends Pharmacol. Sci.

Life Sci.

Brain Res.