Streptomycin blocks the postsynaptic effects of excitatory amino acids on the vestibular system primary afferents

doi:10.1016/0006-8993(91)91537-B

Brain Research

Volume 563, Issues 1–2, 1 November 1991, Pages 221-226

https://doi.org/10.1016/0006-8993(91)91537-B Get rights and content

Abstract

It has been suggested that streptomycin might be an antagonist of the glutamate receptors, and that it selectively blocks quisqualic acid receptors. We studied whether streptomycin blocks the responses to excitatory amino acid agonists on the vestibular system primary afferents, and if it allows us to differentiate between kainate (KA) and quisqualate (QA) receptor mediated responses. The experiments were performed in the axolotl (Ambystoma tigrinum). Intra- and extracellular records of the electrical activity of semicircular canal afferent fibers were obtained. Drugs were applied by pressure ejection in volumes of 20 μl in a 10 ml bath. Streptomycin (0.01–10 mM), induced a dose dependent reversible inhibition of the basal spike discharge of the afferent fibers. This coincided with a reduction in the amplitude of excitatory postsynaptic potentials (EPSP) recorded recorded intracellularly in the afferent fibers. Streptomycin also blocked the excitatory action produced by KA and QA; increasing concentrations of streptomycin produced a rightward shift in the concentration-response curves for both KA and QA. This action persisted even in a high Mg²⁺ (10 mM), low Ca²⁺ (0.09 mM) Ringer solution, indicating its postsynapsic nature. These results show that streptomycin might be a non-selective excitatory amino acid (EAA) receptor antagonist.

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Research reportStreptomycin blocks the postsynaptic effects of excitatory amino acids on the vestibular system primary afferents

Abstract

Eur. J. Pharmacol.

Neuroscience

Biochim. Biophys. Acta

Pharmacol. Biochem. Behav.

Biochem. Pharmacol.

Brain Research

Hear. Res.

Comput. Methods Prog. Biomed.

J. Neurosci. Methods

Brain Research

Brain Research

Life Sci.

Hear. Res.

The early effects of streptomycin on first order vestibular neurons in the gerbil

Pharmacology of the vestibular hair cell afferent fiber synapse in the frog

J. Neurosci.

Voltage dependent block of locust muscle glutamate channels by chlorisondamine

J. Exp. Biol.

The aminoglycoside antibiotic, gentamicin, fails to block increases in miniature endplate potential frequency induced by the sulfhydryl reagent, N-ethylmaleimide, in low calcium solutions

Brain Research

The nature of the neuromuscular block produced by neomycine

Acta Physiol. Scand.

Research report
Streptomycin blocks the postsynaptic effects of excitatory amino acids on the vestibular system primary afferents