Elsevier

Brain Research

Volume 552, Issue 2, 28 June 1991, Pages 330-332
Brain Research

Phosphoinositide hydrolysis linked 5-HT2 receptors in fibroblasts from choroid plexus

https://doi.org/10.1016/0006-8993(91)90099-HGet rights and content

Abstract

A serotonin (5-HT)-mediated phosphoinositide hydrolysis response was characterized in fibroblasts cultured from rabbit choroid plexus. 5-HT elicited a maximum 8-fold increase in [3H]inositol-phosphate ([3H]IP) formation, while the partial agonists, (+)-lysergic acid diethylamide and (−)-1-(4-bromo-2,5-dimethyoxyphenyl)-2-aminopropane caused 2- and 5-fold increases, respectively. Mianserin, ketanserin, and spiperone were equipotent at blocking the 5-HT-mediated response. Thus, agonist and antagonist profiles indicate interactions with 5-HT2 receptors.

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Cited by (13)

  • Serotonin and serotonin receptors in hallucinogen action

    2020, Handbook of Behavioral Neuroscience
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    This chapter will review the receptor interactions that contribute to the behavioral effects of hallucinogens and MDMA. The 5-HT2A receptor is a Gq/11-coupled receptor that is linked to the phosphoinositide hydrolysis signaling cascade (Barker, Burris, & Sanders-Bush, 1991; Nichols & Nichols, 2008). Members of both the indoleamine and phenylalkylamine classes of hallucinogens bind to 5-HT2A receptors with high-affinity.

  • Phospholipase C mediates (±)-1-(2,5-dimethoxy-4-iodophenyl)-2- aminopropane (DOI)-, but not lysergic acid diethylamide (LSD)-elicited head bobs in rabbit medial prefrontal cortex

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    As in the current study, DOI-stimulated PI hydrolysis has previously been blocked by 5-HT2A antagonists in cortical tissue (Edwards et al., 1992; Pierce and Peroutka, 1988). The LSD-stimulated signal was also blocked by ketanserin in cell models, but at 10 to 20 times the LSD concentration (Barker et al., 1991; Egan et al., 1998). One study reported complete blocking of the LSD-stimulated PI hydrolysis in rat cortical slices by ketanserin, but the data were not shown (Sanders-Bush et al., 1988).

  • Receptors for 5-hydroxytryptamine

    1993, New Comprehensive Biochemistry
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This work was supported by ADAMHA research Grants DA 05181 and MH 34007 and NIH training grant GM 07628.

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Special thanks to Marsha Breeding for her assistance with cell cultures.

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