Elsevier

Brain Research

Volume 529, Issues 1–2, 8 October 1990, Pages 214-223
Brain Research

Release of immunoreactive substance P in the spinal cord during development of acute arthritis in the knee joint of the cat: a study with antibody microprobes

https://doi.org/10.1016/0006-8993(90)90830-5Get rights and content

Abstract

In anaesthetized spinal cats, the release of immunoreactive substance P in the spinal cord during development of an acute inflammation in one knee joint was studied with antibody microprobes. The microprobes bore antibodies directed to the C- or N-terminus of substance P. With the normal knee joint, innocuous mechanical stimuli (flexion, pressure) did not result in spinal release of immunoreactive substance P. Following injection of kaolin and carrageenen into a knee, evidence for release of su bstance P following joint stimulation was found in 7 of 10 cats. Such release did not occur for several hours after joint injection and was detected predominantly in the superficial dorsal horn, the dorsal columns and at the dorsal surface of the spinal cord. In some experiments release was detected in the deep dorsal horn and upper ventral horn. Release of immunoreactive substance P required periods of mechanical stimulation such as flexion of, or pressure to, the inflamed joint. The failure to detect central release of substance P from stimulation of normal joints, and the release of substance P, after a delay, from inflamed joints, suggest that the fibres releasing this compound require sensitization by inflammatory mediators before they are exicited by joint stimuli.

Reference (46)

  • KuraishiY. et al.

    Stimulus specificity of peripherally evoked substance P release from the rabbit dorsal horn in situ

    Neuroscience

    (1989)
  • LeahJ.D. et al.

    Coexistence of peptide immunoreactivity in sensory neurons of the cat

    Neuroscience

    (1985)
  • LeeC.M. et al.

    The development and application of a novel N-terminal directed substance P antiserum

    Life Sci.

    (1980)
  • MeyerR.A. et al.

    A novel electrophysiological technique for locating cutaneous nociceptive and chemospecific receptors

    Brain Research

    (1988)
  • MuraseK. et al.

    Substance P augments a persistent slow inward calcium sensitive current in voltage-clamped spinal dorsal horn neurons of the rat

    Brain Research

    (1986)
  • OkuR. et al.

    Release of substance P from the spinal dorsal horn is enhanced in polyarthritic rats

    Neurosci. Lett.

    (1987)
  • PearsonC.M.

    Experimental joint disease. Observations on adjuvant-induced arthritis

    J. Chron. Dis.

    (1963)
  • RandicM. et al.

    Effects of polyclonal and monoclonal antibodies to substance P on slow excitatory transmission in rat spinal dorsal horn

    Brain Research

    (1986)
  • SchoenenJ. et al.

    Histochemical changes of substance P, FRAP, serotonin and succinic dehydrogenase in the spinal cord of rats with adjuvant arthritis

    Life Sci.

    (1985)
  • YakshT.L.

    Substance P release from knee joint afferent terminals; modulation by opioids

    Brain Research

    (1988)
  • BartfaiT. et al.

    Regulation of the release of coexisting neurotransmitters

    Annu. Rev. Pharmacol. Toxicol.

    (1988)
  • BattagliaG. et al.

    Coexistence of glutamate and substance P in dorsal root ganglion neurons of the rat and monkey

    J. Comp. Neurol.

    (1988)
  • CraigA.D. et al.

    The projection of the medial and posterior articular nerves of cat's knee to the spinal cord

    J. Comp. Neurol.

    (1988)
  • Cited by (187)

    • 5.32 - Joint Pain

      2020, The Senses: A Comprehensive Reference: Volume 1-7, Second Edition
    • Antagonism of substance P and perception of breathlessness in patients with chronic obstructive pulmonary disease

      2014, Respiratory Physiology and Neurobiology
      Citation Excerpt :

      Like endogenous opioids, substance P plays a key role in nociception. In response to a noxious stimulus, substance P is released from terminals of sensory nerve fibers that transmit pain impulses from peripheral NK-1 receptors to the CNS (Stein and Lang, 2009; Duggan et al., 1988; Schaible et al., 1990). Like endogenous opioids, substance P and the NK-1 receptor are present in the respiratory system, being found in bronchial smooth muscle, blood vessels, mucous glands, immune cells, and sensory nerves (Kudlacz et al., 1993).

    • Inhibition of ERK phosphorylation by substance P N-terminal fragment decreases capsaicin-induced nociceptive response

      2011, Neuropharmacology
      Citation Excerpt :

      Numerous studies have documented the presence of substance P in sensory afferent fibers in the dorsal root ganglia and the dorsal horn of the spinal cord (Pernow, 1983). Substance P is released from the spinal cord following various nociceptive stimuli (Duggan et al., 1987; Kantner et al., 1985; Schaible et al., 1990) and administrations of capsaicin through peripheral and intrathecal (i.t.) routes (Gamse et al., 1979; Go and Yaksh, 1987). Following release and binding to neurokinin1 (NK1) receptor, the biological action of substance P is terminated by enzymatic degradation (Lee et al., 1981; Matsas et al., 1984; Nyberg and Terenius, 1991).

    • A-995662 [(R)-8-(4-methyl-5-(4-(trifluoromethyl)phenyl)oxazol-2-ylamino)-1, 2,3,4-tetrahydronaphthalen-2-ol], a novel, selective TRPV1 receptor antagonist, reduces spinal release of glutamate and CGRP in a rat knee joint pain model

      2010, Pain
      Citation Excerpt :

      In support, in a cat acute arthritic knee joint model, nociception alone was not sufficient to stimulate SP release. Spinal SP release was only induced after severe stimuli or damaging insults [42]. CGRP [27], glutamate [26,29], and bradykinin [28,38] have been shown to sensitize nociceptors, either directly or indirectly, suggesting that TRPV1 may be sensitized in OA rats.

    View all citing articles on Scopus
    *

    Present address: Physiologisches Institut der Universität Wurzburg, F.R.G.

    **

    Present address: Clinical Pharmacology and Therapeutics Unit, Austin Hospital, Melbourne, Victoria Australia.

    View full text