A modified cupric-silver technique for the impregnation of degenrating neurons and their processes

doi:10.1016/0006-8993(81)90571-0

Brain Research

Volume 208, Issue 2, 16 March 1981, Pages 426-431

https://doi.org/10.1016/0006-8993(81)90571-0 Get rights and content

Abstract

A rapid version of the de Olmos-Ingram cupric-silver technique ^1,3 with higher sensitivity and affinity for degenerative changes is introduced for the staining of mechanically and chemically induced degeneration. Emphasis is laid on induction of degeneration with experimental approaches sparing ‘fibers of passage’.

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Mapping and reconstruction of domoic acid-induced neurodegeneration in the mouse brain
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Domoic acid, a potent neurotoxin and glutamate analog produced by certain species of the marine diatom Pseudonitzschia, is responsible for several human and wildlife intoxication events. The toxin characteristically damages the hippocampus in exposed humans, rodents, and marine mammals. Histochemical studies have identified this, and other regions of neurodegeneration, though none have sought to map all brain regions affected by domoic acid. In this study, mice exposed (i.p.) to 4 mg/kg domoic acid for 72 h exhibited behavioral and pathological signs of neurotoxicity. Brains were fixed by intracardial perfusion and processed for histochemical analysis. Serial coronal sections (50 μm) were stained using the degeneration-sensitive cupric silver staining method of DeOlmos. Degenerated axons, terminals, and cell bodies, which stained black, were identified and the areas of degeneration were mapped onto Paxinos mouse atlas brain plates using Adobe Illustrator CS™. The plates were then combined to reconstruct a 3-dimensional image of domoic acid-induced neurodegeneration using Amira 3.1™ software. Affected regions included the olfactory bulb, septal area, and limbic system. These findings are consistent with behavioral and pathological studies demonstrating the effects of domoic acid on cognitive function and neurodegeneration in rodents.
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A modified cupric-silver technique for the impregnation of degenrating neurons and their processes

Abstract

Brain Research

Brain Research

Analyt. Biochem.

A cupric-silver method for impregnation of terminal axon degeneration and its further use in staining granular argyrophilic neurons

Brain, Behav. Evol.

Silver methods for the impregnation of degenerating axoplasm

Morphological and chemical studies of Nauta-stained degenerating cerebellar and hypothalamic fibers

J. comp. Neurol.

Factors affecting the formation of metallic silver and the binding of silver ions by tissue components

Histochemistry