Elsevier

Biochemical Pharmacology

Volume 40, Issue 6, 15 September 1990, Pages 1399-1403
Biochemical Pharmacology

Induction of cytochrome P450IA1 in rat hepatoma cell by polycyclic hydrocarbons and a dioxin

https://doi.org/10.1016/0006-2952(90)90409-EGet rights and content

Abstract

In this study, the effects of benzo[a]pyrene (BP), 3-methylcholanthrene (3MC) and tetrachlorodibenzofuran (TCDBF) on the expression of the cytochrome P450IA1 gene in the rat hepatoma cell line H4IIE were examined. The initial rate of increase in the steady-state concentration of the mRNA for this gene was similar with each of these inducers; however, the elevated level of this mRNA was more sustained after TCDBF treatment. Nuclear run-off assays suggested that the elevated level of the mRNA was caused principally by an effect upon transcription.

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