The nature of the inhibition in vitro of cerebral glutamine synthetase by the convulsant, methionine sulfoximine☆
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Cited by (31)
Effect of glucose on glutamine metabolism in rat brain slices: A cellular metabolomic study with <sup>13</sup>C NMR
2013, NeuroscienceCitation Excerpt :The appearance of new glutamine, which can be calculated as the difference between the removal of [3-13C]glutamine and the removal of glutamine determined enzymatically, was 16.6 ± 3.4 in the absence of glucose and 37.0 ± 3.2 μmol/g dry weight/h in the presence of glucose (p = 0.0001, Student’s t test for paired data). In order to determine the participation of glutamine synthetase in the appearance of new glutamine when glucose was added to the incubation medium [the participation of glutamine synthetase in the appearance of new glutamine has been already determined when glutamine was used as sole substrate, (El Hage et al., 2011a)], experiments were performed in which rat brain slices were incubated with 5 mM [3-13C]glutamine plus 5 mM unlabeled glucose in the absence and the presence of 2 mM MSO, an irreversible inhibitor of glutamine synthetase (Sellinger and Weiler, 1963; Lamar and Sellinger, 1965). As can be seen in Fig. 2, not only the removal of glutamine measured enzymatically but also the removal of [3-13C]glutamine were increased in the presence of MSO; the [3-13C]glutamine removal was 52.8 and 63.5 μmol/g dry weight/h (p < 0.05) in the absence and the presence of glucose.
Metabolic fate of a high concentration of glutamine and glutamate in rat brain slices: A <sup>13</sup>C NMR study
2011, Neurochemistry InternationalCitation Excerpt :To test if this new glutamine was formed by glutamine synthetase, we first designed experiments to inhibit this enzyme activity. For this, we incubated rat brain slices with glutamate in the absence and the presence of various concentrations of methionine sulfoximine (MSO), an irreversible inhibitor of glutamine synthetase (Lamar and Sellinger, 1965; Sellinger and Weiler, 1963). The results of these experiments revealed that, at all the concentrations (2, 5 and 10 mM) of MSO used, glutamine synthesis from glutamate (measured by both enzymatic and NMR techniques) was virtually completely abolished.
Natural occurrence of methionine sulfoximine in the connaraceae family
1985, Journal of EthnopharmacologyAssociation-dissociation of mammalian brain glutamine synthetase: Effects of metal ions and other ligands
1984, Archives of Biochemistry and BiophysicsGlutamine uptake by isolated rat brain mitochondria
1982, Neuroscience
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Supported in part by Grant H-1525 from the National Institutes of Health and by Institutional Grant 1N-24D from the United States Public Health Service to Tulane University.
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Recipient of a Tulane University Student Research Award.