Endothelin-1 and endothelin-3 stimulate calcium mobilization by different mechanisms in vascular smooth muscle

doi:10.1016/0006-291X(92)90538-V

Biochemical and Biophysical Research Communications

Volume 183, Issue 2, 16 March 1992, Pages 694-700

https://doi.org/10.1016/0006-291X(92)90538-V Get rights and content

Abstract

The mechanisms by which endothelin-1 (ET-1) and endothelin-3 (ET-3) stimulate Ca²⁺ mobilization were investigated in rat aortic smooth muscle cells. Both ET-1 and ET-3 potently stimulated mobilization of Ca²⁺ from intracellular stores, however only ET-1-stimulated Ca²⁺ mobilization appeared to occur as a consequence of an elevation in cellular inositol trisphosphate (IP₃) concentration. Neomycin, an inhibitor of phospholipase C, inhibited both the increase in [³H]IP₃ formation and the mobilization of Ca²⁺ induced by ET-1, however it did not affect Ca²⁺ mobilization induced by ET-3. Together these findings indicate that ET-1 stimulates Ca²⁺ mobilization via an increase in IP₃, whereas the effect of ET-3 appears to be mediated by a separate, IP₃-independent signalling pathway.

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Endothelin-1 and endothelin-3 stimulate calcium mobilization by different mechanisms in vascular smooth muscle

Abstract

Atheroscler

Biochem. Biophys. Res. Commun

Biochem. Biophys. Res. Commun

Biochem. Biophys. Res. Commun

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

J. Biol. Chem

Cell

FEBS LETTS

J. Biol. Chem

J. Biol. Chem

Biochem. Biophys. Res. Commun