Biochemical and Biophysical Research Communications
Comparative partial purification of the active dicarboxylate transport system of rat liver, kidney and heart mitochondria
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Reactive oxygen species production in cardiac mitochondria after complex i inhibition: Modulation by substrate-dependent regulation of the NADH/NAD<sup>+</sup> ratio
2016, Free Radical Biology and MedicineCitation Excerpt :These results, together with substrate-dependent reversibility of NADH/H2O2 production in permeabilized mitochondria, suggest that the actual concentration of substrates/products in the vicinity of reversible matrix enzymes have an important role. Cardiac mitochondria reportedly exhibit dicarboxylate carrier activity that is six times lower compared with liver mitochondria [7]. In addition to relatively low carrier activity, glutamate uptake through glutamate/aspartate antiporter is electrogenic and requires membrane potential [8,9].
Evaluation of molecularity of rate-limiting step of pore formation by antimicrobial peptides studied using mitochondria as a biosensor
2012, Toxicology in VitroCitation Excerpt :The standard RLM concentration in the oximetric cell was 0.25 mg/ml except for experiments on determination of the partition coefficient (Kp) for distribution of TAM between RLM and the incubation medium. SMP were obtained by the simplified method of Saint-Macary and Foucher (1985). Briefly, the RLM preparation was placed in hypotonic medium containing 4 mM Tris (pH 7.4) and 2 mM EDTA for 20 min at 0 °C, and after addition of sucrose to 250 mM the mitoplasts were pelleted by centrifugation at 6000g for 20 min.
A bioenergetic model of the mitochondrial population undergoing permeability transition
2010, Journal of Theoretical BiologyCitation Excerpt :Mitochondria from specific tissue types are phenotypically different and contain different amounts of innermembrane proteins, matrix proteins and lipid types optimized to support their designated function. For example, cardiac mitochondria are reported to possess a less active dicarboxylate carrier than the liver mitochondria (Saint-Macary and Foucher, 1985). This carrier is responsible for the exchange of primarily Pi, malate and succinate.
Cardiolipin and mitochondrial carriers
2009, Biochimica et Biophysica Acta - BiomembranesCitation Excerpt :The isolation of the tricarboxylate carrier from liver mitochondria profited early from the CL interference [25] but it took some time to elucidate the intricacies for separating the tricarboxylate carrier [26]. Isolation of the dicarboxylate carrier from liver mitochondria also involved the use of CL for increasing the yield [27,28–30]. Similarly, the carrier for oxoglutarate isolated from heart [31] and from liver mitochondria [29] depended on the addition of CL for their isolation.