Determination of SRS-A release from guinea-pig lungs by a radioimmunoassay

https://doi.org/10.1016/0006-291X(83)91239-1Get rights and content

Abstract

A sensitive radioimmunoassay for leukotrienes (LTs) has been developed. Rabbits were immunized with a conjugate of LTD4 and bovine serum albumin, prepared by using 1,5-difluoro-2,4-dinitrobenzene as the coupling agent. The assay can detect 0.045 pmol LTD4 at a final plasma dilution of 1:72. 50% displacement of bound 3H-LTD4 was obtained with 0.43±0.03 pmol LTD4. LTC4, LTE4 and LTF4 cross-react 159%, 57% and 85%, respectively, whereas LTB4, 5-HETE and prostaglandins did not. The assay was validated by measuring the antigen-induced release of LTs from sensitized guinea-pig chopped lungs. High correlation (0.9434, p<0.05) was found when LTs were simultaneously determined by this assay and a bioassay on guinea pig ileum.

References (14)

  • R.D. Krell et al.

    Prostaglandins

    (1981)
  • J.A. Lindgren et al.

    FEBS LETT

    (1983)
  • B.S. Tsai et al.

    Prostaglandins

    (1982)
  • K. Bernstrom et al.

    Biochem. Biophys. Res. Commun

    (1982)
  • D. Aharony et al.

    Biochem. Biophys. Acta

    (1982)
  • R.N. Young et al.

    Prostaglandins

    (1982)
  • J.M. Drazen et al.
There are more references available in the full text version of this article.

Cited by (41)

  • Increased expression of endothelial cell adhesion molecules due to mediator release from human foreskin mast cells stimulated by autoantibodies in chronic urticaria sera

    2002, Journal of Investigative Dermatology
    Citation Excerpt :

    The limit of detection of the assay was approximately 1–5 ng of histamine per ml, and the amount of histamine released was expressed as a percentage change of the total histamine present in the unstimulated cells. The leukotriene content in each of the cell supernatants was determined by radioimmunoassay as described previously (Aharony et al, 1983). The leukotriene antibody was diluted in buffered saline (5 mM MES, HEPES adjusted to pH 7.4 with 1 M NaOH) containing 0.1% gelatin.

View all citing articles on Scopus
View full text