Biochemical and Biophysical Research Communications
Interaction of a fluorescent agonist with the membrane-bound acetylcholine receptor from in the millisecond time range: Resolution of an “intermediate” conformational transition and evidence for positive cooperative effects
References (42)
- et al.
FEBS Lett
(1976) - et al.
J. Mol. Biol
(1965) - et al.
J. Mol. Biol
(1971) - et al.
J. Physiol. (Lond.)
(1978) - et al.
FEBS Lett
(1977) - et al.
Biochemistry
(1979) - et al.
J. Physiol. (Lond.)
(1957) - et al.
J. Gen. Physiol
(1975) - et al.
C.R. Acad. Sci. (Paris)
(1977) - et al.
Eur. J. Biochem
(1979)et al.Eur. J. Biochem
(1979)
Eur. J. Biochem
FEBS Lett
J. Gen. Physiol
Biochemistry
Biochemistry
Biochemistry
Biochemistry
Science
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