Crystallographic study of single crystals of mitochondrial coupling factor (BF1) from beef heart

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Abstract

Beef heart mitochondrial coupling factor (BF1) was crystallized from 0.1 M Tris-PO4, pH 7.8, containing 1 mM EDTA, 4 mM ATP and 1.85 M (NH4)2SO4, at 22°C. Single crystals were obtained different from those reported by Spitzberg and Haworth (Biochim. Biophys. Acta 492, 237–240, 1977). The X-ray diffraction pattern reveals an orthorhombic lattice with a = 150 Å, b = 132 Å and c = 180 Å and, according to the absence of reflection, a space group of C2221. The crystal density was determined to 1.19 g·ml−1 and, assuming a molecular weight of 350,000 for BF1, there is only one half of the BF1 molecule in the asymmetric unit cell.

References (19)

  • B.A. Baird et al.

    Biochim. Biophys. Acta

    (1979)
  • A.E. Knowles et al.

    J. Biol. Chem

    (1972)
  • D.H. MacLennan et al.

    J. Biol. Chem

    (1968)
  • B.A. Baird et al.

    J. Biol. Chem

    (1977)
  • H.H. Paradies et al.

    J. Biol. Chem

    (1978)
  • H.H. Paradies et al.

    J. Biol. Chem

    (1979)
  • H.S. Penefsky
  • V. Spitzberg et al.

    Biochim. Biophys. Acta

    (1977)
  • L.L. Horstmann et al.

    J. Biol. Chem

    (1970)
There are more references available in the full text version of this article.

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