Elsevier

Analytical Biochemistry

Volume 149, Issue 1, 15 August 1985, Pages 284-290
Analytical Biochemistry

Determination of malondialdehyde by ion-pairing high-performance liquid chromatography

https://doi.org/10.1016/0003-2697(85)90506-8Get rights and content

Abstract

A method for the analysis of malondialdehyde (MDA) by ion-pairing HPLC is described. The method is direct, no derivitization is required, and sample preparation is minimal. After removal of particulates, the samples are injected directly onto an octadecylsilane column which is eluted with 14% (vv) acetonitrile in 50 mm myristyltrimethylammonium bromide, 1 mm phosphate, pH 6.8. Detection is accomplished by monitoring absorbance at 254 nm or for greater sensitivity at 267 nm. The lower limit for reliable quantitation is 5 pmol MDA and the dynamic range extends to at least 4 nmol MDA. The method has been applied to the quantitation of MDA production during microsomal lipid peroxidation and to an assessment of the stability of MDA in microsomal and urine samples.

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Supported by grants from G. D. Searle & Co. and the National Institutes of Health (CA 22206).

1

L. J. Marnett is a recipient of a Faculty Research Award (FRA 243) from the American Cancer Society.

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