Elsevier

Analytical Biochemistry

Volume 30, Issue 3, September 1969, Pages 339-345
Analytical Biochemistry

A radiochemical method for monoamine oxidase assay

https://doi.org/10.1016/0003-2697(69)90126-2Get rights and content

Abstract

A simple radiochemical method is described for the assay of monoamine oxidase utilizing tritiated tyramine. The synthesis of tyramine-1,2-3H from tyrosine-2,3-3H is reported. Tritium, released by the oxidation of tyramine-1,2-3H by monoamine oxidase, was counted by conventional liquid scintillation techniques. Also reported is the application of this method for the study of a number of parameters which influence the activity of the enzyme, including pH, substrate concentration, and amount of enzyme.

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