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Dynamic models of G-protein coupled receptor dimers: indications of asymmetry in the rhodopsin dimer from molecular dynamics simulations in a POPC bilayer

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Abstract

Based on the growing evidence that G-protein coupled receptors (GPCRs) form homo- and hetero-oligomers, models of GPCR signaling are now considering macromolecular assemblies rather than monomers, with the homo-dimer regarded as the minimal oligomeric arrangement required for functional coupling to the G-protein. The dynamic mechanisms of such signaling assemblies are unknown. To gain some insight into properties of GPCR dimers that may be relevant to functional mechanisms, we study their current structural prototype, rhodopsin. We have carried out nanosecond time-scale molecular dynamics (MD) simulations of a rhodopsin dimer and compared the results to the monomer simulated in the same type of bilayer membrane model composed of an equilibrated unit cell of hydrated palmitoyl-oleoyl-phosphatidyl choline (POPC). The dynamic representation of the homo-dimer reveals the location of structural changes in several regions of the monomeric subunits. These changes appear to be more pronounced at the dimerization interface that had been shown to be involved in the activation process [Proc Natl Acad Sci USA 102:17495, 2005]. The results are consistent with a model of GPCR activation that involves allosteric modulation through a single GPCR subunit per dimer.

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Acknowledgements

We are grateful to Dr. Marc Ceruso for help in setting up the initial dimer simulations, and Dr. Nathalie Basdevant for help with the essential dynamics analysis. This work was supported by NIH grants DA00060, DA012923 (to HW) and DA020032, DA017976 (to MF) from the National Institute on Drug Abuse.

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Authors and Affiliations

  1. Department of Physiology & Biophysics, Weill Medical College of Cornell University, 1300 York Ave, New York, NY, 10021, USA

    Marta Filizola, Simon X. Wang & Harel Weinstein

  2. HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Medical College of Cornell University, New York, NY, 10021, USA

    Harel Weinstein

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  1. Marta Filizola
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  2. Simon X. Wang
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  3. Harel Weinstein
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Correspondence to Marta Filizola or Harel Weinstein.

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Filizola, M., Wang, S.X. & Weinstein, H. Dynamic models of G-protein coupled receptor dimers: indications of asymmetry in the rhodopsin dimer from molecular dynamics simulations in a POPC bilayer. J Comput Aided Mol Des 20, 405–416 (2006). https://doi.org/10.1007/s10822-006-9053-3

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