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Ruthenium red reduces the Ca2+ sensitivity of Ca2+ uptake into cardiac sarcoplasmic reticulum

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Abstract

 Ruthenium red inhibits mitochondrial Ca2+ uptake and is widely used as an inhibitor of ryanodine-sensitive Ca2+ channels that function to release Ca2+ from the sarcoplasmic reticulum (SR) of muscle cells. It also has effects on other Ca2+ channels and ion transporters. To study the effects of ruthenium red on Ca2+ transport into the SR of cardiac muscle cells, fluorescence measurements of Ca2+ uptake into cardiac SR vesicles were made. Ruthenium red significantly decreased the Ca2+ sensitivity of SR uptake in a dose-dependent manner at concentrations ranging from 5 μM to 20 μM. There were no significant effects of ruthenium red on the maximum velocity or the Hill coefficient of SR Ca2+ uptake.

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Received: 14 January 1998 / Received after revision: 12 March 1998 / Accepted: 16 March 1998

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Kargacin, G., Ali, Z. & Kargacin, M. Ruthenium red reduces the Ca2+ sensitivity of Ca2+ uptake into cardiac sarcoplasmic reticulum. Pflügers Arch 436, 338–342 (1998). https://doi.org/10.1007/s004240050641

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  • DOI: https://doi.org/10.1007/s004240050641

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