Abstract
The neuroblastoma × glioma hybrid cell line NH15-CA2 was used to test the effects of two interleukins on neuronal Na+ channels. The cells were cultured in the presence of dibutyryl cAMP and retinoic acid, which yielded a high expression of Na+ channels so that the cells were excitable. Na+ currents were triggered and recorded in the whole-cell recording mode. Comparison of the effects of tetrodotoxin and μ-conotoxin established that the expressed Na+ channels were of the neuronal type. Bath-applied recombinant human interleukin-2 (rIL-2) had a reversible inhibitory effect on the Na+ currents, although to a lesser extent than on muscular Na+ currents (more than 1000 U/ml required for 50% block in NH15-CA2 cells vs 500 U/ml in myoballs). The current/voltage relationship was not affected by the presence of rIL-2, but the steady-state inactivation curve was shifted by –7.7 ± 4.8 mV (mean ± SD, n=18). Recombinant human interleukin-1β (rIL-1β), applied at 1000 U/ml, showed an inhibitory effect on the Na+ currents in about one-third of the cells tested. The mechanism of inhibition was different from that of rIL-2, as rIL-1β seemed to cause a block without affecting voltage dependence or kinetics of the channels.
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Received: 8 March 1996 / Received after revision: 8 May 1996 / Accepted: 12 July 1996
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Hamm, S., Rüdel, R. & Brinkmeier, H. Excitatory sodium currents of NH15-CA2 neuroblastoma x glioma hybrid cells are differently affected by interleukin-2 and interleukin-1β. Pfluegers Arch 433, 160–165 (1996). https://doi.org/10.1007/s004240050262
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DOI: https://doi.org/10.1007/s004240050262